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Modeling The Lifecycle Of Ebola Virus Under Biosafety Level 2 Conditions With Virus-like Particles Containing Tetracistronic Minigenomes
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Modeling The Lifecycle Of Ebola Virus Under Biosafety Level 2 Conditions With Virus-like Particles Containing Tetracistronic Minigenomes

Modeling The Lifecycle Of Ebola Virus Under Biosafety Level 2 Conditions With Virus-like Particles Containing Tetracistronic Minigenomes

DOI:
10.3791/52381-v

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10:11 min

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September 27, 2014

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, , ,
1Laboratory of Virology, Division of Intramural Research,National Institute of Allergy and Infectious Diseases, National Institutes of Health, 2Research Technology Branch, Division of Intramural Research,National Institute of Allergy and Infectious Diseases, National Institutes of Health

Chapters

  • 00:00Title
  • 03:18Step 1: Initial Production of trVLPs
  • 05:41Step 2: Preparation and Infection of Target Cells
  • 07:13Step 3: Harvest of Cells and Determination of Reporter Activity
  • 08:55Results
  • 09:25Conclusions

Summary

Automatic Translation

Automatic Translation

Work with infectious Ebola viruses is restricted to biosafety level 4 laboratories. Tetracistronic minigenome-containing replication and transcription-competent virus like particles (trVLPs) represent a lifecycle modeling system that allows us to safely model multiple infectious cycles under biosafety level 2 conditions, relying exclusively on Ebola virus components.

Tags

Ebola VirusVirus-like ParticlesTetracistronic MinigenomesBiosafety Level 2Virus Lifecycle ModelingReplication And Transcription-competent Virus-like ParticlesVP40GP12VP24MorphogenesisBuddingEntryGenome ReplicationTranscription

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