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Journal / Immunology and Infection / Modellering livscykel Ebola Virus Under…
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Modellering livscykel Ebola Virus Under skyddsnivå 2 Villkor Med virusliknande partiklar innehållande Tetracistronic Minigenomes
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Modellering livscykel Ebola Virus Under skyddsnivå 2 Villkor Med virusliknande partiklar innehållande Tetracistronic Minigenomes

Article DOI: 10.3791/52381-v • 10:11 min • September 27th, 2014
September 27th, 2014 •
1, 1, 2, 1
1Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 2Research Technology Branch, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health

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Arbetet med smitt Ebola-virus är begränsad på biosäkerhet nivå fyra laboratorier. Tetracistronic minigenome innehållande replikation och transkription kompetenta viruslika partiklar (trVLPs) utgör ett livscykelmodelleringssystem som tillåter oss att på ett säkert sätt modellera flera infektionscykler under skyddsnivå 2 villkor, att förlita sig enbart på Ebola viruskomponenter.

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Infektionssjukdomar hemorragiska febrar Viral Mononegavirales Infektioner Ebola-virus filovirus livscykelmodellering systemet minigenome omvänd genetik virusliknande partiklar replikering transkription spirande morphogenesis posten
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