/
/
Sensitive Detection of Proteopathic Seeding Activity with FRET Flow Cytometry
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
- 00:05Title
- 00:55Biological Seed Material Preparation
- 02:29Biosensor Cell Replating, Treating, and Harvesting
- 05:29Förster Resonance Energy Transfer (FRET) Flow Cytometry
- 08:22Data Analysis
- 09:58Results: Representative FRET Analyses
- 11:45Conclusion
Cell-to-cell transfer of protein aggregates, or proteopathic seeds, may underlie the progression of pathology in neurodegenerative diseases. Here, a novel FRET flow cytometry assay is described that enables specific and sensitive detection of seeding activity from recombinant or biological samples.
