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An In Vitro Model for Studying Cellular Transformation by Kaposi Sarcoma Herpesvirus
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JoVE Journal Biology
An In Vitro Model for Studying Cellular Transformation by Kaposi Sarcoma Herpesvirus

An In Vitro Model for Studying Cellular Transformation by Kaposi Sarcoma Herpesvirus

DOI:
10.3791/54828-v

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09:53 min

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August 25, 2017

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, , , ,
1Division of Pediatric Infectious Diseases,University of Minnesota Medical School, 2Vaccine and Gene Therapy Institute,Oregon Health and Science University

Chapters

  • 00:05Title
  • 01:09KSHV Stock Preparation
  • 03:15Transduction of Endothelial Cells with E6 and E7 Papillomavirus Genes
  • 05:41Infection of Transduced Endothelial Cells with KSHV
  • 07:38Results: Phenotypic Changes in KSHV-infected Endothelial Cells
  • 09:15Conclusion

Summary

Automatic Translation

Automatic Translation

Kaposi sarcoma (KS) is a tumor induced by infection with the oncogenic virus human herpesvirus-8/KS herpesvirus (HHV-8/KSHV). The endothelial cell culture model described here is uniquely suited for studying the mechanisms by which KSHV transforms host cells.

Tags

In Vitro ModelCellular TransformationKaposi Sarcoma HerpesvirusKSHVEndothelial CellsMalignant TransformationViral OncogenesisKaposi SarcomaPathological AngiogenesisBCBL-1 CellsPMAViral Particle IsolationUltracentrifugation

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