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Generation of Genetically Modified Mice through the Microinjection of Oocytes
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Generation of Genetically Modified Mice through the Microinjection of Oocytes
DOI:

10:19 min

June 15, 2017

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Chapters

  • 00:05Title
  • 00:53Synthesis of CRISPER Components
  • 04:21Injection Mix and Pronuclear and Cytoplasmic Injections
  • 06:31Reimplantation
  • 08:21Results: Microinjection of Oocytes to Generate Genetically Modified Mice
  • 09:49Conclusion

Summary

Automatic Translation

The microinjection of mouse oocytes is commonly used for both classic transgenesis (i.e., the random integration of transgenes) and CRISPR-mediated gene targeting. This protocol reviews the latest developments in microinjection, with a particular emphasis on quality control and genotyping strategies.

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