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Patterning Bioactive Proteins or Peptides on Hydrogel Using Photochemistry for Biological Applications
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Patterning Bioactive Proteins or Peptides on Hydrogel Using Photochemistry for Biological Applications
DOI:

09:19 min

September 15, 2017

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Chapters

  • 00:05Title
  • 00:52Preparation of Materials for Hydrogel Synthesis
  • 01:57Preparation of Sterile Glass Slide Molds for Hydrogel Formation
  • 03:04Hydrogel Formation
  • 04:06Protein Patterning
  • 05:18Cell Seeding onto Hydrogels
  • 06:58Results: Endothelial Cells Respond to VEGF Patterned Hydrogels
  • 08:31Conclusion

Summary

Automatic Translation

In this method, we use photopolymerization and click chemistry techniques to create protein or peptide patterns on the surface of polyethylene glycol (PEG) hydrogels, providing immobilized bioactive signals for the study of cellular responses in vitro.

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