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Ultrahigh Resolution Mouse Optical Coherence Tomography to Aid Intraocular Injection in Retinal Gene Therapy Research
JoVE Journal
Medicine
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JoVE Journal Medicine
Ultrahigh Resolution Mouse Optical Coherence Tomography to Aid Intraocular Injection in Retinal Gene Therapy Research
DOI:

10:10 min

November 02, 2018

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Chapters

  • 00:04Title
  • 00:45Subretinal Injection
  • 02:37Injection Site and Subretinal Bleb Mapping
  • 04:03High Resolution Spectral-Domain Optical Coherence Tomography (HR-SD-OCT) Outer Retinal Degeneration Evaluation
  • 07:47Results: Representative Outer Retinal Change Quantification and Post-Subretinal Injection 3D Mapping
  • 08:54Conclusion

Summary

Automatic Translation

Here we demonstrate a novel approach to using high resolution spectral-domain optical coherence tomography (HR-SD-OCT) to assist delivery of gene therapy agents into the subretinal space, assess its areal coverage, and characterize photoreceptor vitality.

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