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A Ligated Intestinal Loop Model in Anesthetized Specific Pathogen Free Chickens to Study Clostridium Perfringens Virulence
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A Ligated Intestinal Loop Model in Anesthetized Specific Pathogen Free Chickens to Study Clostridium Perfringens Virulence

A Ligated Intestinal Loop Model in Anesthetized Specific Pathogen Free Chickens to Study Clostridium Perfringens Virulence

DOI:
10.3791/57523-v

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09:21 min

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October 11, 2018

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, , ,
1Department of Clinical Sciences, Faculty of Veterinary Medicine,Université de Montréal, 2University of Bristol

Chapters

  • 00:04Title
  • 00:56Preparations
  • 01:42Extraction of the Small Intestines from the Abdominal Cavity
  • 03:11Fabricating Intestinal Loops and Injecting them with Pathogens
  • 04:58Completing the Procedure and Collecting Results
  • 06:44Results: Identifying Tissue Damage Caused by Pathogens or by Vascular Congestion
  • 08:02Conclusion

Summary

Automatic Translation

Automatic Translation

Here we present a protocol to surgically create 'intestinal ligated loops' in chicken small intestines. This procedure allows for the comparison of multiple Clostridium perfringens strains' virulence in situ in a single host. This method markedly decreases the number of chickens usually necessary for similar in vivo experiments.

Tags

Ligated Intestinal Loop ModelAnesthetized ChickensClostridium PerfringensVirulenceChicken Necrotic EnteritisIntestinal PathogensSurgical ProcedureAnesthesiaSkin PreparationAbdominal IncisionIntestine ExteriorizationJejunum

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