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A Ligated Intestinal Loop Model in Anesthetized Specific Pathogen Free Chickens to Study Clostridium Perfringens Virulence
JoVE Journal
Medicine
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JoVE Journal Medicine
A Ligated Intestinal Loop Model in Anesthetized Specific Pathogen Free Chickens to Study Clostridium Perfringens Virulence
DOI:

09:21 min

October 11, 2018

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Chapters

  • 00:04Title
  • 00:56Preparations
  • 01:42Extraction of the Small Intestines from the Abdominal Cavity
  • 03:11Fabricating Intestinal Loops and Injecting them with Pathogens
  • 04:58Completing the Procedure and Collecting Results
  • 06:44Results: Identifying Tissue Damage Caused by Pathogens or by Vascular Congestion
  • 08:02Conclusion

Summary

Automatic Translation

Here we present a protocol to surgically create 'intestinal ligated loops' in chicken small intestines. This procedure allows for the comparison of multiple Clostridium perfringens strains' virulence in situ in a single host. This method markedly decreases the number of chickens usually necessary for similar in vivo experiments.

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