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Journal / Neuroscience / 一种简便、可再生的新分离大鼠脑微血管膜样品制备方法…
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JoVE Journal Neuroscience
一种简便、可再生的新分离大鼠脑微血管膜样品制备方法
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一种简便、可再生的新分离大鼠脑微血管膜样品制备方法

Article DOI: 10.3791/57698-v • 07:13 min • May 7th, 2018
May 7th, 2018 •
*1, *1, 1, 1
1Department of Pharmacology, College of Medicine, University of Arizona, Tucson
* These authors contributed equally

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本文介绍了一种分离大鼠脑微血管及制备膜样品的方法。该协议具有明显的优势, 生产丰富的微血管样品与可接受的蛋白质产量从个别动物。样品可用于大脑微血管内皮的健壮蛋白质分析。

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神经科学 问题 135 血脑屏障 脑微血管 葡聚糖分离 差异离心 内皮细胞 膜蛋白 分子药理学 转运体 紧密连接 西方印迹
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