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JoVE Journal Bioengineering
En dråbe mikrofluid tilgang og Microsphere-PCR-amplifikation til enkeltstrenget DNA amplikoner
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En dråbe mikrofluid tilgang og Microsphere-PCR-amplifikation til enkeltstrenget DNA amplikoner

Article DOI: 10.3791/57703-v • 11:40 min • November 14th, 2018
November 14th, 2018 •
*1, *2, 2, 3, 3, 1, 2,3, 1
1College of Natural Sciences, Chungbuk National University, 2Department of Biomedical Engineering, Inje University, 3Institute of Digital Anti-Aging Healthcare, Inje University
* These authors contributed equally

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Dette værk indeholder en metode til fremstilling af droplet-baserede mikrofluid platforme og anvendelsen af polyacrylamid mikrokugler for microsphere-PCR-amplifikation. Microsphere-PCR-metoden gør det muligt at opnå enkeltstrenget DNA amplikoner uden adskillelse dobbelt-strenget DNA.

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Bioteknologi sag 141 Copolymerization mikrofluid kanal strøm-fokusere Microsphere-PCR ssDNA forstærkning asymmetriske PCR
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