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Journal / Developmental Biology / 纳米粒子介导的 siRNA…
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Developmental Biology

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JoVE Journal Developmental Biology
纳米粒子介导的 siRNA 基因沉默在斑马鱼心脏中的表达
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纳米粒子介导的 siRNA 基因沉默在斑马鱼心脏中的表达

Article DOI: 10.3791/58054-v • 09:12 min • July 29th, 2018
July 29th, 2018 •
1, 2, 1, 1, 2, 1
1Beijing Key Laboratory of Cardiometabolic Molecular Medicine, State Key Laboratory of Natural and Biomimetic Drugs, Institute of Molecular Medicine, Peking University, 2Department of Biomedical Engineering, College of Engineering, Peking University

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在成年斑马鱼器官中发展有条件基因剔除或有效基因击倒仍是一项重大挑战。在这里, 我们报告了在成年斑马鱼心脏执行纳米粒子介导的 siRNA 基因沉默的协议, 从而为研究斑马鱼和其他模型生物体中的成年器官提供了一种新的功能损失方法。

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发育生物学 137 期 斑马鱼 成人心脏再生 纳米微粒 siRNA 基因沉默 发育生物学 遗传学
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