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Morphometric Protocol for the Objective Assessment of Blastocyst Behavior During Vitrification and Warming Steps
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Chapters
- 00:04Title
- 00:41Blastocyst Recording During Equilibration, Cross-Sectional Area Measurement, Data Editing and Creation of a Line Diagram
- 03:27Blastocyst Time-Lapse Re-Expansion Recording, Measurements, Data Editing and Creation of Line Diagram
- 06:09Results: Representative Blastocoel Contraction and Re-Expansion Analyses
- 07:49Conclusion
Here we present a time-lapse morphometric protocol to follow the intensity of blastocyst shrinkage and re-expansion during previtrification interventions and post-warming recovery. The application of the protocol is possible in in vitro fertilization laboratories equipped with time-lapse microscopes and is recommended in the development of an optimal blastocyst vitrification method.
