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Journal / Biology / LDL Kolesterol opptaksanalyse bruke levende celle …
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Biology

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LDL Kolesterol opptaksanalyse bruke levende celle Imaging analyse med cellen helseovervåking
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LDL Kolesterol opptaksanalyse bruke levende celle Imaging analyse med cellen helseovervåking

Article DOI: 10.3791/58564-v • 08:45 min • November 17th, 2018
November 17th, 2018 •
*1,2, *1,3, 4, 4,5, 6, 1,2,7,8
1Interdisciplinary Stem Cell Institute, University of Miami Leonard M. Miller School of Medicine, 2Department of Medicine, Division of Cardiology, University of Miami Leonard M. Miller School of Medicine, 3Department of Molecular and Cellular Pharmacology, University of Miami Leonard M. Miller School of Medicine, 4Dr. John T. Macdonald Foundation Department of Human Genetics, University of Miami Leonard M. Miller School of Medicine, 5John P. Hussman Institute for Human Genomics, University of Miami Leonard M. Miller School of Medicine, 6Department of Medicine, Division of Endocrinology, Metabolism and Endocrinology and the Diabetes Research Institute, University of Miami Leonard M. Miller School of Medicine, 7Vascular Biology Institute, University of Miami Leonard M. Miller School of Medicine, 8Peggy and Harold Katz Family Drug Discovery Center, University of Miami Leonard M. Miller School of Medicine
* These authors contributed equally

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Denne protokollen gir en effektiv tilnærming til måling LDL Kolesterol opptak med sanntid tilstrømningen priser ved bruk av en levende celle imaging system i ulike celletyper. Denne teknikken gir en plattform til skjermen farmakologiske aktiviteten av stoffer påvirker LDL tilstrømningen under overvåking av cellen morfologi og dermed potensielle cytotoksisitet.

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Biologi problemet 141 lav tetthet lipoprotein LDLR LDL tilstrømningen LDL opptak Dynasore Simvastatin rekombinant PCSK9 dynamin lipider kolesterol åreforkalkning.
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