Journal
/
Cancer Research
/
Evaluation of Biomarkers in Glioma by Immunohistochemistry on Paraffin-Embedded 3D Glioma Neurosphere Cultures
JoVE Journal
Cancer Research
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Cancer Research
Evaluation of Biomarkers in Glioma by Immunohistochemistry on Paraffin-Embedded 3D Glioma Neurosphere Cultures

Evaluation of Biomarkers in Glioma by Immunohistochemistry on Paraffin-Embedded 3D Glioma Neurosphere Cultures

DOI:
10.3791/58931-v

•

06:32 min

•

January 09, 2019

•

, , , , , ,
1Department of Neurosurgery,University of Michigan Medical School, 2Department of Cell & Developmental Biology,University of Michigan, 3INIBIOLP, Histology B-Pathology B, School of Medicine,UNLP

Chapters

  • 00:04Title
  • 00:38Fixation of Tumor NS
  • 01:21Paraffin Embedding of Tumor NS
  • 01:51Sectioning of Paraffin-Embedded NS
  • 03:23Immunohistochemistry (IHC) of Paraffin-Embedded NS
  • 05:31Results: NS Generated, Analyzed via Bioluminescence, and Stained for Glioma Biomarkers
  • 06:10Conclusion

Summary

Automatic Translation

Automatic Translation

Neurospheres grown as 3D cultures constitute a powerful tool to study glioma biology. Here we present a protocol to perform immunohistochemistry while maintaining the 3D structure of glioma neurospheres through paraffin embedding. This method enables the characterization of glioma neurosphere properties such as stemness and neural differentiation.

Tags

3D Glioma Neurosphere CulturesImmunohistochemistryParaffin-embeddedBiomarkersMicrotome Sectioning

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

A Protocol for Rapid Post-mortem Cell Culture of Diffuse Intrinsic Pontine Glioma (DIPG)

Four-color Fluorescence Immunohistochemistry of T-cell Subpopulations in Archival Formalin-fixed, Paraffin-embedded Human Oropharyngeal Squamous Cell Carcinoma Samples

Determination of Protein Expression Level in Cultured Cells by Immunocytochemistry on Paraffin-embedded Cell Blocks

Elastic Staining on Paraffin-embedded Slides of pT3N0M0 Gastric Cancer Tissue

Assessing Cell Viability and Death in 3D Spheroid Cultures of Cancer Cells

Real-Time Monitoring of Human Glioma Cell Migration on Dorsal Root Ganglion Axon-Oligodendrocyte Co-Cultures

A Rapid Screening Workflow to Identify Potential Combination Therapy for GBM using Patient-Derived Glioma Stem Cells

Co-culture of Glutamatergic Neurons and Pediatric High-Grade Glioma Cells Into Microfluidic Devices to Assess Electrical Interactions

Live-3D-Cell Immunocytochemistry Assays of Pediatric Diffuse Midline Glioma

Digital Spatial Profiling for Characterization of the Microenvironment in Adult-Type Diffusely Infiltrating Glioma

Read Article