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Identifying Cell Surface Markers of Primary Neural Stem and Progenitor Cells by Metabolic Labeling of Sialoglycan
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Identifying Cell Surface Markers of Primary Neural Stem and Progenitor Cells by Metabolic Labeling of Sialoglycan
DOI:

11:39 min

September 07, 2019

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Chapters

  • 00:05Title
  • 01:16Preparation of Mouse Endothelial Culture in Permeable Support Inserts
  • 01:55Set-up of Neural-endothelial Co-culture and Ac4ManNAz Labeling System
  • 03:25Immunofluorescent Staining of Sialoglycoproteins in Expanded Primary NSPCs and Differentiated Neurons
  • 05:25Purification of Sialoglycoproteins from Expanded Primary NSPCs and Differentiated Neurons
  • 08:45Results: Identifying Cell Surface Markers by Metabolic Labeling of Sialoglycan
  • 10:47Conclusion

Summary

Automatic Translation

Presented here is a protocol that combines an in vitro neural-endothelial co-culture system and metabolic incorporation of sialoglycan with bioorthogonal functional groups to expand primary neural stem and progenitor cells and label their surface sialoglycoproteins for imaging or mass-spectrometry analysis of cell surface markers.

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