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Enhancing the Engraftment of Human Induced Pluripotent Stem Cell-derived Cardiomyocytes via a Transient Inhibition of Rho Kinase Activity
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1Department of Biomedical Engineering, School of Medicine, School of Engineering,University of Alabama at Birmingham, 2Division of Cardiovascular Diseases, School of Medicine, School of Engineering,University of Alabama at Birmingham, 3Department of Cardiac Surgery,The Second Xiangya Hospital of Central South University
Chapters
- 00:04Title
- 01:13hiPSC-CMs Purification, Small Molecule Pre-treatment, Myocardial Infarction, and Cell Transplantation
- 03:42Calcium Transient and Contractility Recording
- 05:21Results: Increased Engraftment of Transplanted hiPSC-CM Preconditioned with the ROCK Inhibitor Y-27632 in a Murine MI Model
- 07:11Conclusion
In this protocol, we demonstrate and elaborate on how to use human induced pluripotent stem cells for cardiomyocyte differentiation and purification, and further, on how to improve its transplantation efficiency with Rho-associated protein kinase inhibitor pretreatment in a mouse myocardial infarction model.
