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Immunology and Infection

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JoVE Journal Immunology and Infection
An in vitro batch-kultur modell for å anslå virkningene av interventional regimer på Human avføring bakterieflora
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An in vitro batch-kultur modell for å anslå virkningene av interventional regimer på Human avføring bakterieflora

Article DOI: 10.3791/59524-v • 07:15 min • July 31st, 2019
July 31st, 2019 •
*1,2,3, *1,2, *1,2, 1,2, 3,4, 5, 1,2
1Department of Internal Medicine- Molecular Medicine, Wake Forest School of Medicine, 2Department of Microbiology and Immunology, Wake Forest School of Medicine, 3Department of Food Science and Technology, Isfahan University of Technology, 4Research Institute for Biotechnology and Bioengineering, College of Agriculture, Isfahan University of Technology, 5Department of Geriatrics and Gerontology, Wake Forest School of Medicine
* These authors contributed equally

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Denne protokollen beskriver en in vitro batch-kultur gjæring system av menneskelig avføring bakterieflora, ved hjelp inulin (en velkjent prebiotiske og en av de mest studerte bakterieflora modulatorer) for å demonstrere bruken av dette systemet i estimering effekter av spesifikke av avførings bakterieflora sammensetning og metabolske aktiviteter.

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Immunologi og infeksjon anaerob batch kultur kolon gjæring in vitro bakterieflora mikroflora Short-Chain fettsyrer pH
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