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Journal
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Immunology and Infection
/
评估介入疗法对人体粪便微生物群影响的体内批处理培养模型
JoVE Journal
Immunology and Infection
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JoVE Journal
Immunology and Infection
An In Vitro Batch-culture Model to Estimate the Effects of Interventional Regimens on Human Fecal Microbiota
Please note that all translations are automatically generated.
Click here for the English version.
评估介入疗法对人体粪便微生物群影响的体内批处理培养模型
DOI:
10.3791/59524-v
•
07:15 min
•
July 31, 2019
•
Shokouh Ahmadi*
1,2,3
,
Shaohua Wang*
1,2
,
Ravinder Nagpal*
1,2
,
Rabina Mainali
2
,
Sabihe Soleimanian-Zad
4
,
Dalane Kitzman
,
Hariom Yadav
2
1
Department of Internal Medicine- Molecular Medicine
,
Wake Forest School of Medicine
,
2
Department of Microbiology and Immunology
,
Wake Forest School of Medicine
,
3
Department of Food Science and Technology
,
Isfahan University of Technology
,
4
Research Institute for Biotechnology and Bioengineering, College of Agriculture
,
Isfahan University of Technology
,
5
Department of Geriatrics and Gerontology
,
Wake Forest School of Medicine
Chapters
00:04
Title
01:42
Preparation for Fermentation Experiment
03:52
Fermentation and Sampling
05:01
Results: The Effect of Prebiotic Treatment
06:28
Conclusion
Summary
Automatic Translation
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Automatic Translation
该协议描述了人类粪便微生物群的体外批量培养发酵系统,使用硫素(一种众所周知的益生菌和研究最广泛的微生物群调制器之一)来证明该系统在估计特定效果时的使用粪便微生物群成分和代谢活动的干预措施。
Tags
Gut Microbiome
In Vitro Batch-culture Model
Fecal Microbiota
Microbiome Modulators
Probiotics
Prebiotics
Diets
Drugs
Short-chain Fatty Acids
Anaerobic Conditions
Aseptic Conditions
Fermentation Media
Anaerobic Dilution Solution
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