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Journal / Immunology and Infection / Salvataggio del virus zoka ricombinante da un…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Salvataggio del virus zoka ricombinante da un clone cDNA cromosoma artificiale batterico
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Salvataggio del virus zoka ricombinante da un clone cDNA cromosoma artificiale batterico

Article DOI: 10.3791/59537-v • 08:10 min • June 24th, 2019
June 24th, 2019 •
1, 1, 1, 2, 1
1Department of Microbiology and Immunology, University of Rochester Medical Center, 2Department of Molecular and Cell Biology, Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid

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La recente epidemia del virus zika sottolinea l'importanza di stabilire approcci genetici inversi per sviluppare vaccini e/o strategie terapeutiche. Qui, descriviamo il protocollo per salvare un virus infettivo ricombinante zika da un clone cDNA a lunghezza intera assemblato in un cromosoma artificiale batterico sotto il controllo del promotore riciclo-precoce umano.

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Immunologia e infezione numero 148 Arbovirus flavivirus virus zika genetica inversa cloni infettivi cDNA a lunghezza intera cromosoma artificiale batterico trasfezione cDNA salvataggio virus
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