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Detecting and Characterizing Protein Self-Assembly In Vivo by Flow Cytometry
JoVE Journal
Biology
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JoVE Journal Biology
Detecting and Characterizing Protein Self-Assembly In Vivo by Flow Cytometry
DOI:

05:58 min

July 17, 2019

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Chapters

  • 00:04Title
  • 00:56Saccharomyces cerevisiae Preparation
  • 02:04Photoconversion and Distributed Amphifluoric Fluorescence Resonance Energy Transfer (DAmFRET) Data Collection
  • 02:51Data Analysis
  • 03:32Results: Representative In Vivo Protein Self-Assembly Detection and Characterization
  • 05:26Conclusion

Summary

Automatic Translation

This article describes a FRET-based flow cytometry protocol to quantify protein self-assembly in both S. cerevisiae and HEK293T cells.

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