Journal
/
Biology
/
Detecting and Characterizing Protein Self-Assembly In Vivo by Flow Cytometry
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
Detecting and Characterizing Protein Self-Assembly In Vivo by Flow Cytometry

Detecting and Characterizing Protein Self-Assembly In Vivo by Flow Cytometry

DOI:
10.3791/59577-v

•

05:58 min

•

July 17, 2019

•

, , , ,
1Stowers Institute for Medical Research, 2Department of Molecular and Integrative Physiology,The University of Kansas School of Medicine

Chapters

  • 00:04Title
  • 00:56Saccharomyces cerevisiae Preparation
  • 02:04Photoconversion and Distributed Amphifluoric Fluorescence Resonance Energy Transfer (DAmFRET) Data Collection
  • 02:51Data Analysis
  • 03:32Results: Representative In Vivo Protein Self-Assembly Detection and Characterization
  • 05:26Conclusion

Summary

Automatic Translation

Automatic Translation

This article describes a FRET-based flow cytometry protocol to quantify protein self-assembly in both S. cerevisiae and HEK293T cells.

Tags

Protein Self-assemblyFlow CytometryDAmFRETFRETAcceptor SignalYeast CultureInduction MediumPhoto ConversionImaging Flow CytometerFluorescence Positive Gate

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

IP-FCM: Immunoprecipitation Detected by Flow Cytometry

Flow Cytometry Purification of Mouse Meiotic Cells

Optimized Staining and Proliferation Modeling Methods for Cell Division Monitoring using Cell Tracking Dyes

Discovering Protein Interactions and Characterizing Protein Function Using HaloTag Technology

In Vivo 4-Dimensional Tracking of Hematopoietic Stem and Progenitor Cells in Adult Mouse Calvarial Bone Marrow

Cytosolic Calcium Measurements in Renal Epithelial Cells by Flow Cytometry

Analysis of Cell Suspensions Isolated from Solid Tissues by Spectral Flow Cytometry

Measuring Endoreduplication by Flow Cytometry of Isolated Tuber Protoplasts

Detecting Protein Subcellular Localization by Green Fluorescence Protein Tagging and 4′,6-Diamidino-2-phenylindole Staining in Caenorhabditis elegans

High-throughput Measurement of Dictyostelium discoideum Macropinocytosis by Flow Cytometry

Read Article