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Determining Bile Duct Density in the Mouse Liver
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Determining Bile Duct Density in the Mouse Liver
DOI:

07:35 min

April 30, 2019

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Chapters

  • 00:04Title
  • 00:36Mouse Liver Tissue Collection
  • 01:19Fixation, Paraffin Embedding, and Liver Block Sectioning
  • 02:35Immunohistochemistry for Wide-spectrum Cytokeratin (wsCK) and αSMA
  • 04:08Imaging and Quantification of Bile Ducts (BDs)
  • 05:27Results: Determining BD to PV Ratio
  • 07:00Conclusion

Summary

Automatic Translation

We present a rather simple and sensitive method for accurate quantification of bile duct density in the mouse liver. This method can aid in determining the effects of genetic and environmental modifiers and the effectiveness of potential therapies in mouse models of biliary diseases.

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