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Determining Bile Duct Density in the Mouse Liver
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Developmental Biology
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JoVE Journal Developmental Biology
Determining Bile Duct Density in the Mouse Liver

Determining Bile Duct Density in the Mouse Liver

DOI:
10.3791/59587-v

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07:35 min

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April 30, 2019

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,
1Program in Developmental Biology,Baylor College of Medicine, 2Medical Scientist Training Program (MSTP),Baylor College of Medicine, 3Department of Molecular and Human Genetics,Baylor College of Medicine

Chapters

  • 00:04Title
  • 00:36Mouse Liver Tissue Collection
  • 01:19Fixation, Paraffin Embedding, and Liver Block Sectioning
  • 02:35Immunohistochemistry for Wide-spectrum Cytokeratin (wsCK) and αSMA
  • 04:08Imaging and Quantification of Bile Ducts (BDs)
  • 05:27Results: Determining BD to PV Ratio
  • 07:00Conclusion

Summary

Automatic Translation

Automatic Translation

We present a rather simple and sensitive method for accurate quantification of bile duct density in the mouse liver. This method can aid in determining the effects of genetic and environmental modifiers and the effectiveness of potential therapies in mouse models of biliary diseases.

Tags

Bile Duct DensityMouse LiverLiver DiseaseGenetic ModifiersBile Duct DevelopmentImmunohistochemistryParaffin EmbeddingLiver SectioningAntigen Retrieval

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