Journal
/
Cancer Research
/
Flow Cytometric Analysis of Mitochondrial Reactive Oxygen Species in Murine Hematopoietic Stem and Progenitor Cells and MLL-AF9 Driven Leukemia
JoVE Journal
Cancer Research
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Cancer Research
Flow Cytometric Analysis of Mitochondrial Reactive Oxygen Species in Murine Hematopoietic Stem and Progenitor Cells and MLL-AF9 Driven Leukemia

Flow Cytometric Analysis of Mitochondrial Reactive Oxygen Species in Murine Hematopoietic Stem and Progenitor Cells and MLL-AF9 Driven Leukemia

DOI:
10.3791/59593-v

•

09:44 min

•

September 05, 2019

•

,
1Blood Cell Development and Function Program,Fox Chase Cancer Center

Chapters

  • 00:05Title
  • 01:46Mitochondrial ROS Fluorogenic Dye Staining
  • 04:41Lineage Antibody Staining
  • 06:04Flow Cytometry Acquisition and Analysis
  • 07:24Results: Flow Cytometry Gating Strategies and Mitochondrial ROS Levels
  • 08:54Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a method for using multiparameter flow cytometry to detect mitochondrial reactive oxygen species (ROS) in murine healthy hematopoietic stem and progenitor cells (HSPCs) and leukemia cells from a mouse model of acute myeloid leukemia (AML) driven by MLL-AF9.

Tags

Flow CytometryMitochondrial Reactive Oxygen SpeciesHematopoietic Stem CellsHematopoietic Progenitor CellsAcute Myeloid LeukemiaMLL-AF9Redox StateMetabolism

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

A Detailed Protocol for Characterizing the Murine C1498 Cell Line and its Associated Leukemia Mouse Model

Comprehensive Protocol to Sample and Process Bone Marrow for Measuring Measurable Residual Disease and Leukemic Stem Cells in Acute Myeloid Leukemia

Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up

Immunoglobulin Gene Sequence Analysis In Chronic Lymphocytic Leukemia: From Patient Material To Sequence Interpretation

Assessment of the Metabolic Profile of Primary Leukemia Cells

Flow Cytometric Detection of Newly-formed Breast Cancer Stem Cell-like Cells After Apoptosis Reversal

Spatial and Temporal Control of Murine Melanoma Initiation from Mutant Melanocyte Stem Cells

Evaluating Cell Death Using Cell-Free Supernatant of Probiotics in Three-Dimensional Spheroid Cultures of Colorectal Cancer Cells

Two Flow Cytometric Approaches of NKG2D Ligand Surface Detection to Distinguish Stem Cells from Bulk Subpopulations in Acute Myeloid Leukemia

Flow Cytometric Analysis of Apoptotic Biomarkers in Actinomycin D-Treated SiHa Cervical Cancer Cells

Read Article