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Coincubation Assay for Quantifying Competitive Interactions between Vibrio fischeri Isolates
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Coincubation Assay for Quantifying Competitive Interactions between Vibrio fischeri Isolates
DOI:

07:43 min

July 22, 2019

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Chapters

  • 00:04Title
  • 00:28Preparation of Each Bacterial Strain for Incubation
  • 01:21Coincubation of Strains
  • 02:54Serial Dilution for Starting Inoculum
  • 04:37Taking T Final Measurements
  • 05:36Results: Quantifying Competitive Interactions Between the Strains
  • 07:26Conclusion

Summary

Automatic Translation

Bacteria encode diverse mechanisms for engaging in interbacterial competition. Here, we present a culture-based protocol for characterizing competitive interactions between bacterial isolates and how they impact the spatial structure of a mixed population.

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