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Journal / Neuroscience / Generation af Neuro kugler fra blandede primære…
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JoVE Journal Neuroscience
Generation af Neuro kugler fra blandede primære hippocampus og kortikale neuroner isoleret fra E14-E16 Sprague Dawley rotte embryo
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Generation af Neuro kugler fra blandede primære hippocampus og kortikale neuroner isoleret fra E14-E16 Sprague Dawley rotte embryo

Article DOI: 10.3791/59800-v • 12:22 min • August 31st, 2019
August 31st, 2019 •
1,2, 1, 1, 1, 1,2
1Organic and Medicinal Chemistry Division, CSIR-Indian Institute of Chemical Biology, 2Academy of Scientific and Innovative Research (AcSIR)

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Præsenteret her er en protokol for spontan generation af Neuro kugler beriget i neurale stamceller fra høj densitet belagte neuroner. Under det samme eksperiment, når neuroner er belagt med en lavere tæthed, protokollen resulterer også i langvarige primære rotte neuron kulturer.

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Neurovidenskab Neuro kugler primær neuron kultur hippocampus neuroner kortikale neuroner blandet kultur Sprague Dawley embryo neurale stamceller NPCs
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