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In Vivo Immunofluorescence Localization for Assessment of Therapeutic and Diagnostic Antibody Biodistribution in Cancer Research
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Cancer Research
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JoVE Journal Cancer Research
In Vivo Immunofluorescence Localization for Assessment of Therapeutic and Diagnostic Antibody Biodistribution in Cancer Research

In Vivo Immunofluorescence Localization for Assessment of Therapeutic and Diagnostic Antibody Biodistribution in Cancer Research

DOI:
10.3791/59810-v

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08:53 min

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September 16, 2019

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,
1Apoptosis, Cancer and Development Laboratory – Equipe labellisée ‘La Ligue’, LabEx DEVweCAN, Centre de Cancérologie de Lyon,INSERM U1052-CNRS UMR5286, Centre Léon Bérard, 2Department of Radiology/Molecular Imaging Program at Stanford, School of Medicine,Stanford University

Chapters

  • 00:04Title
  • 00:50Intravenous Injection of Specific and Nonspecific Antibody Agents
  • 02:27Collection and Preparation of Target Tumor Tissues
  • 03:53Ex Vivo Staining Protocol
  • 06:07Results: IVIL Shows Varying Biodistribution Patterns of Anti-B7-H3 and Anti-Netrin-1 Antibodies in Murine Mammary Tumors In Vivo Compared to Respective Control Antibodies
  • 07:50Conclusion

Summary

Automatic Translation

Automatic Translation

The in vivo immunofluorescence localization (IVIL) method can be used to examine in vivo biodistribution of antibodies and antibody conjugates for oncological purposes in living organisms using a combination of in vivo tumor targeting and ex vivo immunostaining methods.

Tags

In Vivo Immunofluorescence LocalizationIVILTherapeutic AntibodyDiagnostic AntibodyBiodistributionCancer ResearchTumor GrowthTail Vein Inoculation

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