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Screening and Identification of Small Peptides Targeting Fibroblast Growth Factor Receptor2 using a Phage Display Peptide Library
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Screening and Identification of Small Peptides Targeting Fibroblast Growth Factor Receptor2 using a Phage Display Peptide Library
DOI:

07:32 min

September 30, 2019

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Chapters

  • 00:04Title
  • 00:54First Round of Biopanning: Screening Phage Clones that Bind to the Extracellular Domain of FGFR2
  • 02:26Titer Determination of the Eluted Phages
  • 03:28Phage Amplification
  • 05:38Results: Screening and Identification of Small Peptides Targeting Fibroblast Growth Factor Receptor2
  • 06:38Conclusion

Summary

Automatic Translation

Herein, we present a detailed protocol for screening small peptides that bind to FGFR2 using a phage display peptide library. We further analyze the affinity of the selected peptides toward FGFR2 in vitro and its ability to suppress cell proliferation.

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