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Monitoring Extracellular pH in Cross-Kingdom Biofilms using Confocal Microscopy
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Monitoring Extracellular pH in Cross-Kingdom Biofilms using Confocal Microscopy
DOI:

10:26 min

January 30, 2020

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Chapters

  • 00:05Title
  • 01:17Cultivation of Cross-kingdom Biofilms
  • 03:35Ratiometric pH Imaging
  • 05:07Calibration of the Ratiometric Dye
  • 06:15Digital Image Analysis
  • 08:38Results: pH Ratiometry in Cross-kingdom Biofilms Exposed to Glucose
  • 09:40Conclusion

Summary

Automatic Translation

The protocol describes the cultivation of cross-kingdom biofilms consisting of Candida albicans and Streptococcus mutans and presents a confocal microscopy-based method for the monitoring of extracellular pH inside these biofilms.

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