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JoVE Journal Neuroscience
Isolering av region-spesifikke Mikroglia fra en voksen mus Brain halvkule for Deep single-celle RNA sekvensering
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Isolering av region-spesifikke Mikroglia fra en voksen mus Brain halvkule for Deep single-celle RNA sekvensering

Article DOI: 10.3791/60347-v • 09:49 min • December 3rd, 2019
December 3rd, 2019 •
1, 1
1Department of Neurobiology, Stanford University School of Medicine

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Vi tilbyr en protokoll for isolering av mikroglia fra ulike dissekert regioner av en voksen mus hjerne halvkule, etterfulgt av semi-automatisert bibliotek forberedelse for dyp enkelt celle RNA sekvensering av full lengde transcriptomes. Denne metoden vil bidra til å belyse funksjonell heterogenitet av mikroglia i helse og sykdom.

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Nevrovitenskap mikroglia isolasjon Brain region fluorescens-aktivert celle sortering Single-celle RNA sekvensering neuroimmunology heterogenitet
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