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Identifying Inhibitors of the HBx-DDB1 Interaction Using a Split Luciferase Assay System
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Identifying Inhibitors of the HBx-DDB1 Interaction Using a Split Luciferase Assay System

Identifying Inhibitors of the HBx-DDB1 Interaction Using a Split Luciferase Assay System

DOI:
10.3791/60652-v

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05:55 min

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December 21, 2019

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, ,
1Department of Gastroenterology, Graduate School of Medicine,The University of Tokyo, 2Research Fellow of Japan Society for the Promotion of Science

Chapters

  • 00:04Title
  • 01:22Cell Preparation
  • 03:48Compound Screening
  • 04:26Results: Split Luciferase Assay
  • 04:56Conclusion

Summary

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Here, we present a method for screening anti-hepatitis B viral agents that inhibit the HBx-DDB1 interaction using a split luciferase assay system. This system allows easy detection of protein-protein interactions and is suitable for identifying inhibitors of such interactions.

Tags

HBX-DDB1 InteractionSplit Luciferase AssayViral TranscriptionCccDNAProtein-protein InteractionsDrug TargetsAntiviral TherapyHPVHEK293T CellsTransfection

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