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Analysis of Non-Human Primate Pancreatic Islet Oxygen Consumption
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Analysis of Non-Human Primate Pancreatic Islet Oxygen Consumption
DOI:

07:12 min

December 18, 2019

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Chapters

  • 00:05Title
  • 01:12Spheroid Microplate Preparation
  • 02:03Sensor Cartridge Hydration
  • 02:36Sensor Cartridge Preparation
  • 03:00Pancreatic Islet Transfer
  • 04:19Oxygen Consumption Assay
  • 05:06Results: Representative Pancreatic Islet Oxygen Consumption Analyses
  • 06:09Conclusion

Summary

Automatic Translation

This protocol demonstrates the accurate and reproducible measurement of oxygen consumption in non-human primate pancreatic islets. The islet loading techniques and coating of the microplate provide a framework for efficient measurement of respiration in other types of cultured spheroids.

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