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Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice
JoVE Journal
Genetics
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JoVE Journal Genetics
Use of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice
DOI:

06:46 min

April 02, 2020

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Chapters

  • 00:05Introduction
  • 00:35In Vitro Fertilization
  • 02:23Embryo Cryopreservation
  • 03:13Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) Reagent Assembly
  • 04:19CRISPR Electroporation
  • 05:09Results: Representative Knockout Mouse Generation Efficiency and Mosaic Rate Evaluation
  • 06:09Conclusion

Summary

Automatic Translation

Here, we present a modified method for cryopreservation of one-cell embryos as well as a protocol that couples the use of freeze-thawed embryos and electroporation for the efficient generation of genetically modified mice.

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