Zaman atlamalı Mikroskopi Sökme Aktin İzlenmesi


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Kueh, H. Y. Monitoring Actin Disassembly with Time-lapse Microscopy. J. Vis. Exp. (1), e66, doi:10.3791/66 (2006).

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  1. Videoda gösterildiği gibi bir akış odası oluşturun. Parafilm mühür sıkı olduğundan emin olun ve yıkanmış lamelleri kullanabilirsiniz.
  2. 5-10 'odasında inkübe aktin bağlayıcı ajan.
  3. Engelleyici bir protein ile cam lamel non-spesifik bağlayıcı siteleri engellemek.
  4. Odanın içinde tampon polimerize G-aktin akan polimerize aktin.
  5. Solukluk fazla tampon akan unpolymerized aktin.

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1 Comment

  1. What is the second pair of shorter parafilm strips for? Why do you polymerize in the chamber and how? G-actin in polymerization buffer. Is't that F-actin? What is the actin binding agent and blocking protein and their buffers and their concentrations?

    Posted by: Anonymous
    July 28, 2009 - 2:41 PM

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