Zaman atlamalı Mikroskopi Sökme Aktin İzlenmesi

Published 11/08/2006
1 Comment
  CITE THIS  SHARE 
Biology

Your institution must subscribe to JoVE's Biology section to access this content.

Fill out the form below to receive a free trial or learn more about access:

Welcome!

Enter your email below to get your free 10 minute trial to JoVE!





By clicking "Submit", you agree to our policies.

 

Summary

Cite this Article

Copy Citation

Kueh, H. Y. Monitoring Actin Disassembly with Time-lapse Microscopy. J. Vis. Exp. (1), e66, doi:10.3791/66 (2006).

Please note that all translations are automatically generated.

Click here for the english version. For other languages click here.

Abstract

Protocol

  1. Videoda gösterildiği gibi bir akış odası oluşturun. Parafilm mühür sıkı olduğundan emin olun ve yıkanmış lamelleri kullanabilirsiniz.
  2. 5-10 'odasında inkübe aktin bağlayıcı ajan.
  3. Engelleyici bir protein ile cam lamel non-spesifik bağlayıcı siteleri engellemek.
  4. Odanın içinde tampon polimerize G-aktin akan polimerize aktin.
  5. Solukluk fazla tampon akan unpolymerized aktin.

Subscription Required. Please recommend JoVE to your librarian.

Comments

1 Comment

  1. What is the second pair of shorter parafilm strips for? Why do you polymerize in the chamber and how? G-actin in polymerization buffer. Is't that F-actin? What is the actin binding agent and blocking protein and their buffers and their concentrations?

    Reply
    Posted by: Anonymous
    July 28, 2009 - 2:41 PM

Post a Question / Comment / Request

You must be signed in to post a comment. Please or create an account.

Video Stats