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Neutrophil Isolation Protocol
JoVE Journal
Biology
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JoVE Journal Biology
Neutrophil Isolation Protocol
DOI:

07:12 min

July 23, 2008

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Chapters

  • 00:00Introduction
  • 00:08Equipment
  • 00:24Materials
  • 01:24Preparation of HBSS/HSA
  • 02:06Blood sample
  • 02:38Stages of neutrophil isolation
  • 02:53First separation: acquire neutrophils
  • 04:56Second separation: refine neutrophil layer
  • 05:39Third separation: lysing the red blood cells
  • 06:27Fourth separation: resuspending the neutrophils

Summary

Automatic Translation

Neutrophils are among the first cells to arrive on the site of inflammatory immune response, and their functions and mechanisms have been studied extensively in vitro. We demonstrate a standard density gradient separation method to isolate human neutrophils from whole blood using commercially available separation media.

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