Protocols for Microapplicator-assisted Infection of Lepidopteran Larvae with Baculovirus



In this video, we demonstrate two microapplicator techniques used to infect of lepidopteran larvae with baculovirus in order to determine insecticidal efficiency.

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Li, H., Sparks, W., Bonning, B. Protocols for Microapplicator-assisted Infection of Lepidopteran Larvae with Baculovirus. J. Vis. Exp. (18), e889, doi:10.3791/889 (2008).


Baculoviruses are widely used both as protein expression vectors and as insect pest control agents. . This video shows how lepidopteran larvae can be infected with microapplicator techniques in the gut with baculovirus polyhedra and in the hemolymph with budded virus. This accompanying Springer Protocols section provides an overview of the baculovirus lifecycle and use of baculoviruses as insecticidal agents. Formulation and application of baculoviruses for pest control purposes are described elsewhere.


Please visit Springer Protocols to learn more about the engineering baculovirus as an insecidical agent and the microapplicator techniques used in this assay.


The authors have nothing to disclose.



  1. I would like to know who sells the syringe-aplicator like you show in this video Thank you MV Miranda

    Posted by: Anonymous
    December 19, 2008 - 8:54 AM
  2. The one used in these protocols was a Burkard Microprocessor Controller Pax-100.    I hope that will help you in your search.   W. O. Sparks

    Posted by: Anonymous
    January 29, 2009 - 10:42 AM

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