In JoVE (10)
- Use of Animal Model of Sepsis to Evaluate Novel Herbal Therapies
- Imaging Glioma Initiation In Vivo Through a Polished and Reinforced Thin-skull Cranial Window
- Imaging pHluorin-tagged Receptor Insertion to the Plasma Membrane in Primary Cultured Mouse Neurons
- Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography
- Towards Biomimicking Wood: Fabricated Free-standing Films of Nanocellulose, Lignin, and a Synthetic Polycation
- Vascular Balloon Injury and Intraluminal Administration in Rat Carotid Artery
- Methods for Characterizing the Co-development of Biofilm and Habitat Heterogeneity
- Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples
- Generation of Integration-free Induced Pluripotent Stem Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors
- Dissection of Larval Zebrafish Gonadal Tissue
Other Publications (5)
- Biochemical and Biophysical Research Communications
- Journal of Psychopharmacology (Oxford, England)
- Journal of Ethnopharmacology
- The International Journal of Neuroscience
- Conference Proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference
Articles by Wei Zhang in JoVE
Use of Animal Model of Sepsis to Evaluate Novel Herbal Therapies Wei Li1, Shu Zhu1, Yusong Zhang1, Jianhua Li1, Andrew E. Sama1, Ping Wang1, Haichao Wang1 1The Feinstein Institute for Medical Research, North Shore – LIJ Health System Sepsis refers to a systemic inflammatory response syndrome resulting from a microbial infection, and can be simulated by a surgical technique termed cecal ligation and puncture (CLP). Here we describe a method to use CLP-induced animal model to screen medicinal herbs for therapeutic agents.
Imaging Glioma Initiation In Vivo Through a Polished and Reinforced Thin-skull Cranial Window Lifeng Zhang*1, Andree Lapierre*1, Brittany Roy1, Maili Lim1, Jennifer Zhu1, Wei Wang1, Stephen B. Sampson1, Kyuson Yun1, Bonnie Lyons1, Yun Li1, Da-Ting Lin1 1The Jackson Laboratory By combining a polished and reinforced thin-skull (PoRTS) cranial window and glioblastoma (GBM) cell injection, we can observe glioma initiation and growth from injected GBM cells in the brain of a live mouse longitudinally.
Imaging pHluorin-tagged Receptor Insertion to the Plasma Membrane in Primary Cultured Mouse Neurons Yun Li1, Brittany D. Roy1, Wei Wang1, Lifeng Zhang1, Stephen B. Sampson1, Da-Ting Lin1 1The Jackson Laboratory By tagging the extracellular domains of membrane receptors with superecliptic pHluorin, and by imaging these fusion receptors in cultured mouse neurons, we can directly visualize individual vesicular insertion events of the receptors to the plasma membrane. This technique will be instrumental in elucidating the molecular mechanisms governing receptor insertion to the plasma membrane.
Integrated Photoacoustic Ophthalmoscopy and Spectral-domain Optical Coherence Tomography Wei Song*1,2, Qing Wei*1, Shuliang Jiao3, Hao F. Zhang1,4 1Department of Biomedical Engineering, Northwestern University, 2Department of Physics, Harbin Institute of Technology, 3Department of Ophthalmology, University of Southern California, 4Department of Ophthalmology, Northwestern University Photoacoustic ophthalmology (PAOM), an optical-absorption-based imaging modality, provides the complementary evaluation of the retina to the currently available ophthalmic imaging technologies. We report the using of PAOM integrated with spectral-domain optical coherence tomography (SD-OCT) for simultaneous multimodal retinal imaging in rats.
Towards Biomimicking Wood: Fabricated Free-standing Films of Nanocellulose, Lignin, and a Synthetic Polycation Karthik Pillai1,2,3, Fernando Navarro Arzate2,4, Wei Zhang2,5, Scott Renneckar1,2,5,6 1Institute for Critical Technology and Applied Science, Virginia Tech, 2Macromolecules and Interfaces Institute, Virginia Tech, 3Institute for Food Safety and Health, Illinois Institute of Technology- Moffett Campus, 4Wood, Cellulose, and Paper Research Department, University of Guadalajara, 5Department of Sustainable Biomaterials, Virginia Tech, 6Sustainable Nanotechnology Interdisciplinary Graduate Education Program, Virginia Tech The objective of this research was to form synthetic plant cell wall tissue using layer-by-layer assembly of nanocellulose fibrils and isolated lignin assembled from dilute aqueous suspensions. Surface measurement techniques of quartz crystal microbalance and atomic force microscopy were used to monitor the formation of the polymer-polymer nanocomposite material.
Vascular Balloon Injury and Intraluminal Administration in Rat Carotid Artery Wei Zhang1, Mohamed Trebak1 1Nanobioscience, State University of New York College of Nanoscale Science and Engineering (SUNY CNSE) This protocol uses a balloon catheter to cause an intraluminal injury on the rat carotid artery and henceforth elicit neointimal hyperplasia. This is a well-established model for studying the mechanisms of vascular remodeling in response to injury. It is also widely used to determine the validity of potential therapeutic approaches.
Methods for Characterizing the Co-development of Biofilm and Habitat Heterogeneity Xiaobao Li1, Jisun L. Song2, Alessandro Culotti1, Wei Zhang1, David L. Chopp3, Nanxi Lu1, Aaron I. Packman1 1Department of Civil and Environmental Engineering, Northwestern University, 2Department of Chemical and Biological Engineeering, Northwestern University, 3Department of Applied Mathematics and Engineering Sciences, Northwestern University Biofilms have complex interactions with their surrounding environment. To comprehensively investigate biofilm-environment interactions, we present here a series of methods to create heterogeneous chemical environment for biofilm development, to quantify local flow velocity, and to analyze mass transport in and around biofilm colonies.
Sheathless Capillary Electrophoresis–Mass Spectrometry for Metabolic Profiling of Biological Samples Wei Zhang1, M. Can Gulersonmez1, Thomas Hankemeier1, Rawi Ramautar1 1Division of Analytical Biosciences, Leiden Academic Center for Drug Research, Leiden University A protocol for metabolic profiling of biological samples by capillary electrophoresis–mass spectrometry using a sheathless porous tip interface design is presented.
Generation of Integration-free Induced Pluripotent Stem Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors Wei Wen1, Jian-Ping Zhang1, Wanqiu Chen2, Cameron Arakaki2, Xiaolan Li1,2, David Baylink2, Gary D. Botimer3, Jing Xu1, Weiping Yuan1, Tao Cheng1,4,5,6,7, Xiao-Bing Zhang1,2 1State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Disease Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, 2Division of Regenerative Medicine, Department of Medicine, Loma Linda University, 3Department of Orthopaedic Surgery, Loma Linda University, 4Center for Stem Cell Medicine, Chinese Academy of Medical Sciences, 5Department of Stem Cell & Regenerative Medicine, Peking Union Medical College, 6Collaborative Innovation Center for Cancer Medicine, 7Tianjin Key Laboratory of Blood Cell Therapy and Technology This protocol describes a detailed method for efficient generation of integration-free iPSCs from human adult peripheral blood cells. With the use of four oriP/EBNA-based episomal vectors to express the reprogramming factors, KLF4, MYC, BCL-XL, or OCT4 and SOX2, thousands of iPSC colonies can be obtained from 1 mL of peripheral blood.
Dissection of Larval Zebrafish Gonadal Tissue Xinjian Wang*1, Sijie Chen*1, Wei Zhang1, Yiyuan Ren1, Quan Zhang1, Gang Peng1 1Institutes of Brain Science, State Key Laboratory of Medical Neurobiology and Collaborative Innovation Center for Brain Science, Fudan University Here, we present a protocol for isolating gonadal tissue of larval zebrafish, which will facilitate investigations of zebrafish sex differentiation and maintenance.
Other articles by Wei Zhang on PubMed
Identification and Fine Mapping of IgG and IgE Epitopes in Ovomucoid Biochemical and Biophysical Research Communications. Apr, 2002 | Pubmed ID: 11944924 Ovomucoid is a major allergen in hen egg white which causes a serious IgE-mediated food allergy reaction. This study determined eight IgG epitopes, 5-11 amino acids in length, and nine IgE epitopes, 5-16 amino acids in length, within the primary sequence in ovomucoid using arrays of overlapping peptides synthesized on cellulose membranes. Pooled sera from eight egg-allergic patients were used to probe the membrane. We also analyzed the amino acids that are critical for antibody binding by substituting a single amino acid within each epitope. Mutational analysis of the epitopes indicated that charged amino acids (aspartic acid, glutamic acid, and lysine) and some hydrophobic (leucine, phenylalanine, and glycine) and polar (serine, threonine, tyrosine, and cystein) amino acids were important for antibody binding. These results provide useful information for the molecular design necessary to reduce the allergenicity of ovomucoid, and a better understanding of structure-function relationships of allergic epitopes in food proteins.
10-Hydroxy-2-decenoic Acid from Royal Jelly: a Potential Medicine for RA Journal of Ethnopharmacology. Mar, 2010 | Pubmed ID: 20138211 Rheumatoid arthritis synovial fibroblasts (RASFs) are known to produce matrix metalloproteinases (MMPs) and cause joint destruction. The purpose of this study is to develop a potential medicine for rheumatoid arthritis (RA).
Autosomal Recessive Posterior Column Ataxia with Retinitis Pigmentosa Caused by Novel Mutations in the FLVCR1 Gene The International Journal of Neuroscience. May, 2014 | Pubmed ID: 24628582 Posterior column ataxia with retinitis pigmentosa (PCARP) is an autosomal recessive disorder characterized by severe sensory ataxia, muscle weakness and atrophy, and progressive pigmentary retinopathy. Recently, mutations in the FLVCR1 gene were described in four families with this condition. We investigated the molecular basis and studied the phenotype of PCARP in a new family. The proband is a 33-year-old woman presented with sensory polyneuropathy and retinitis pigmentosa (RP). The constellation of clinical findings with normal metabolic and genetic evaluation, including mitochondrial DNA (mtDNA) analysis and normal levels of phytanic acid and vitamin E, prompted us to seek other causes of our patient's condition. Sequencing of FLVCR1 in the proband and targeted mutation testing in her two affected siblings revealed two novel variants, c.1547G>A (p.R516Q) and c.1593+5_+8delGTAA predicted, respectively, to be highly conserved throughout evolution and affecting the normal splicing, therefore, deleterious. This study supports the pathogenic role of FLVCR1 in PCARP and expands the molecular and clinical spectra of PCARP. We show for the first time that nontransmembrane domain (TMD) mutations in the FLVCR1 can cause PCARP, suggesting different mechanisms for pathogenicity. Our clinical data reveal that impaired sensation can be part of the phenotypic spectrum of PCARP. This study along with previously reported cases suggests that targeted sequencing of the FLVCR1 gene should be considered in patients with severe sensory ataxia, RP, and peripheral sensory neuropathy.
Chair Rise Transfer Detection and Analysis Using a Pendant Sensor: An Algorithm for Fall Risk Assessment in Older People Conference Proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual Conference. Aug, 2014 | Pubmed ID: 25570333 Falls result in substantial disability, morbidity, and mortality among older people. Early detection of fall risks and timely intervention can prevent falls and injuries due to falls. Simple field tests, such as repeated chair rise, are used in clinical assessment of fall risks in older people. Development of on-body sensors introduces potential beneficial alternatives for traditional clinical methods. In this article, we present a pendant sensor based chair rise detection and analysis algorithm for fall risk assessment in older people. The recall and the precision of the transfer detection were 85% and 87% in standard protocol, and 61% and 89% in daily life activities. Estimation errors of chair rise performance indicators: duration, maximum acceleration, peak power and maximum jerk were tested in over 800 transfers. Median estimation error in transfer peak power ranged from 1.9% to 4.6% in various tests. Among all the performance indicators, maximum acceleration had the lowest median estimation error of 0% and duration had the highest median estimation error of 24% over all tests. The developed algorithm might be feasible for continuous fall risk assessment in older people.