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Protein Kinase C-delta Inhibitor Peptide Formulation using Gold Nanoparticles
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1Latner Thoracic Surgery Research Laboratories, Toronto General Hospital Research Institute,University Health Network, 2Respiratory Medicine Research Laboratory, Institute of Translation Medicine, Shanghai General Hospital,Shanghai Jiaotong University, 3Institute of Medical Science, Faculty of Medicine,University of Toronto
Chapters
- 00:04Title
- 01:02Preparation of Peptide Solutions
- 02:39Formulation of GNP/PKCi and Assessment of GNP/PKCi Hybrid Solubility
- 04:13Results: Analysis of the Gold Nanoparticle Protein Kinase C-delta Inhibitor
- 05:07Conclusion
We have previously used a gold nanoparticle peptide hybrid to intravenously deliver a synthetic peptide, protein kinase C-delta inhibitor, which reduced ischemia-reperfusion-induced acute lung injury. Here we show the detailed protocol of the drug formulation. Other intracellular peptides can be formulated similarly.
