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Monitoring Plasmide Replicatie in Live zoogdiercellen over meerdere generaties door fluorescentie microscopie
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Monitoring Plasmide Replicatie in Live zoogdiercellen over meerdere generaties door fluorescentie microscopie

Article DOI: 10.3791/4305-v • 12:34 min • December 13th, 2012
December 13th, 2012 •
1, 1, 1
1Department of Oncology, University of Wisconsin - Madison

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Werkwijze voor het observeren individuele DNA-moleculen in levende cellen beschreven. De techniek is gebaseerd op de binding van een fluorescent gelabeld lac repressor eiwit bindingsplaatsen ontworpen in het DNA van belang. Deze methode kan worden aangepast aan vele recombinant DNA volgen in levende cellen in de tijd.

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Genetics Molecular Biology Cellular Biology Genomics DNA synthese DNA partitionering plasmiden lac operator lac repressor zoogdiercellen fluorescentiemicroscopie
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