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A semi-High-gjennomstrømning tilpasning av NADH-koblet ATPase analysen for screening små molekyl hemmere

Article DOI: 10.3791/60017-v • 10:28 min • August 17th, 2019
August 17th, 2019 •
1,2, 1,2, 3, 2, 1,2
1Department of Molecular Medicine, The Scripps Research Institute, 2Department of Neuroscience, The Scripps Research Institute, 3Laboratory of Molecular Physiology, NHLBI, National Institutes of Health

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En nikotinamid adenine dinucleotide (NADH)-koblet ATPase analysen har blitt tilpasset semihigh gjennomstrømning screening av små molekyl myosin hemmere. Denne kinetisk analysen kjøres i et 384-brønn mikroplate format med totalt reaksjons volum på bare 20 μL per brønn. Plattformen bør være aktuelt for nesten alle ADP produserende enzym.

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Biokjemi ATPase analysen NADH fluorescens semi høy gjennomstrømming screening hemmende konstant myosin
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