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Developmental Biology

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Een semi-High-throughput imaging methode en data visualisatie Toolkit om C. elegans embryonale ontwikkeling te analyseren
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Een semi-High-throughput imaging methode en data visualisatie Toolkit om C. elegans embryonale ontwikkeling te analyseren

Article DOI: 10.3791/60362-v • 06:49 min • October 29th, 2019
October 29th, 2019 •
1,2,3, 1,2, 1,2, 1,4,5, 1,6, 1,7, 1,2, 1,2, 1,2
1Ludwig Institute for Cancer Research, San Diego, 2Department of Cellular and Molecular Medicine, University of California, San Diego, 3Recursion Pharmaceuticals, 4Biomedical Sciences Graduate Program, University of California, San Diego, 5Cell Biology Section, National Institute of Dental and Craniofacial Research, National Institutes of Health, 6Department of Biology, San Diego State University, 7Developmental and Stem Cell Biology Graduate Program, University of California, San Francisco

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Dit werk beschrijft een semi-High-throughput protocol dat gelijktijdige 3D time-lapse beeldvorming van embryogenese in 80 – 100 C. elegans embryo's in een enkele nachtelijke run toestaat. Daarnaast worden tools voorbeeld verwerking en visualisatie meegeleverd om de gegevensanalyse te stroomlijnen. De combinatie van deze methoden met aangepaste reporter stammen maakt gedetailleerde controle van embryogenese.

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Ontwikkelingsbiologie uitgave 152 C. elegans embryo High-content Imaging embryo Genesis beeldverwerking data visualisatie ontwikkeling embryonale ontwikkeling semi-hoge doorvoer
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