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Genetisk modifisering av cyanobakterier av Bøyning bruke CyanoGate Modular kloning Toolkit

Article DOI: 10.3791/60451-v • 08:25 min • October 31st, 2019
October 31st, 2019 •
*1,2,3, *1,2, *1,2, 2,3, 1,2
1Institute of Molecular Plant Sciences, School of Biological Sciences, University of Edinburgh, 2Centre for Synthetic and Systems Biology, University of Edinburgh, 3Institute of Quantitative Biology, Biochemistry and Biotechnology, School of Biological Sciences, University of Edinburgh
* These authors contributed equally

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Her presenterer vi en protokoll som beskriver hvordan jeg) monterer en selv-replikere vektor bruker CyanoGate modulære kloning Toolkit, II) innføre vektoren i en cyanobacterial vert ved Bøyning, og III) karakterisere transgene cyanobakterier stammer ved hjelp av en plate leser eller strømnings flowcytometri.

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Biologi Flow flowcytometri fluorescens Golden Gate plate leser Synechocystis Sp. PCC 6803 Synechococcus elongatus UTEX 2973 verktøykasse forbigående uttrykk
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