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Journal / Immunology and Infection / Studie av dendritisk cellutveckling av kort…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Studie av dendritisk cellutveckling av kort hårnål RNA-medierad gen knockdown i en hematopoetisk stam och progenitor cellinje in vitro
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Studie av dendritisk cellutveckling av kort hårnål RNA-medierad gen knockdown i en hematopoetisk stam och progenitor cellinje in vitro

Article DOI: 10.3791/62730-v • 06:12 min • March 7th, 2022
March 7th, 2022 •
1, 2, 1
1Graduate Institute of Immunology, National Taiwan University College of Medicine, 2Department of Pathology, Division of Microbiology and Immunology, University of Utah

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Här tillhandahåller vi ett protokoll för screening potentiella transkription faktorer inblandade i utvecklingen av dendritiska cell (DC) med hjälp av lentiviral transduktion av shRNA för att erhålla stabil knockdown cellinjer för in vitro DC differentiering.

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Immunologi och infektion nummer 181 utveckling dendritisk cell knockdown stamceller hematopoiesis transkriptionsfaktorer
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