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JoVE Journal
Immunology and Infection

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Analysis of Human Natural Killer Cell Metabolism

Analysis of Human Natural Killer Cell Metabolism

Article DOI: 10.3791/61466-v
June 22nd, 2020


Summary June 22nd, 2020

In this paper, we describe a method to measure glycolysis and mitochondrial respiration in primary human Natural Killer (NK) cells isolated from peripheral blood, at rest or following IL15-induced activation. The protocol described could be easily extended to primary human NK cells activated by other cytokines or soluble stimuli.


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