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JoVE Journal
Immunology and Infection

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Analysis of Human Natural Killer Cell Metabolism

Analysis of Human Natural Killer Cell Metabolism

Article DOI: 10.3791/61466-v 09:03 min June 22nd, 2020
June 22nd, 2020



In this paper, we describe a method to measure glycolysis and mitochondrial respiration in primary human Natural Killer (NK) cells isolated from peripheral blood, at rest or following IL15-induced activation. The protocol described could be easily extended to primary human NK cells activated by other cytokines or soluble stimuli.



Human Natural Killer Cell Metabolism Activation Oxygen Consumption PH Changes Extracellular Flux Analyzer High Throughput Screens Glycolysis Respiration Lymphocyte Separation Medium Blood Centrifuge Mononuclear Cells PBS NK Separation Buffer
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