Methods to create human 3D tumor tissues as test systems are described. These technologies are based on a decellularized Biological Vascularized Scaffold (BioVaSc), primary human cells and a tumor cell line, which can be cultured under static as well as under dynamic conditions in a flow bioreactor.
Moll, C., Reboredo, J., Schwarz, T., Appelt, A., Schürlein, S., Walles, H., Nietzer, S. Tissue Engineering of a Human 3D in vitro Tumor Test System. J. Vis. Exp. (78), e50460, doi:10.3791/50460 (2013).