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Developmental Biology

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JoVE Journal Developmental Biology
Identifikation og isolering af burstdannende enhed og kolonidannende enhed erythroid-forfædre fra musevæv ved flowcytometri
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Identifikation og isolering af burstdannende enhed og kolonidannende enhed erythroid-forfædre fra musevæv ved flowcytometri

Article DOI: 10.3791/64373-v • 08:31 min • November 4th, 2022
November 4th, 2022 •
1, 1, 1, 1
1Department of Molecular, Cell and Cancer Biology, UMASS Chan Medical School

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Her beskriver vi en ny flowcytometrisk metode til prospektiv isolering af tidlige burstdannende enhedserythroid (BFU-e) og kolonidannende enhedserythroide (CFU-e) forfædre direkte fra frisk museknoglemarv og milt. Denne protokol, udviklet baseret på enkeltcelle transkriptomiske data, er den første til at isolere alle vævets erythroidforfædre med høj renhed.

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Udviklingsbiologi udgave 189 Erythropoiesis flowcytometri FACS CFU-e BFU-e erythroidforfædre
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