JoVE Journal

Cancer Research

JoVE Cancer Research includes experimental approaches and methods to understand, detect, treat, and prevent cancer.

1 2 3 4 5 6 7 8 9…14 › 334 VIDEO ARTICLES

Functional Assessment of BRCA1 variants using CRISPR-Mediated Base Editors

Ji-Eun See^1,2, Ha Rim Shin^1,2, Gayoung Jang^1,2, Jiyeon Kweon^1,2, Yongsub Kim^1,2

¹Department of Biomedical Sciences, Asan Medical Institute of Convergence Science and Technology, Asan Medical Center, University of Ulsan College of Medicine, ²Stem Cell Immunomodulation Research Center, University of Ulsan College of Medicine

JoVE 61557

Co-culture of Glioblastoma Stem-like Cells on Patterned Neurons to Study Migration and Cellular Interactions

Joris Guyon¹, Pierre-Olivier Strale^2,3, Irati Romero-Garmendia⁴, Andreas Bikfalvi¹, Vincent Studer*², Thomas Daubon*⁴

¹University Bordeaux, INSERM, LAMC, U1029, 33600, Pessac, France, ²Univ. Bordeaux, CNRS, Interdisciplinary Institute for Neuroscience, IINS, UMR 5297, Bordeaux, France, ³Alvéole, Bordeaux, France, ⁴University Bordeaux, CNRS, IBGC, UMR5095, 33000, Bordeaux, France

JoVE 62213

Methods Collection

Analysis of Side Population in Solid Tumor Cell Lines

Xiaoli Dong¹, Yingying Wei¹, Tao Xu¹, Xiaoyue Tan^1,2,3, Na Li^1,2,3

¹School of Medicine, Nankai University, ²Tianjin Key Laboratory of Tumour Microenvironment and Neurovascular Regulation, ³Collaborative Innovation Center for Biotherapy, Nankai University

JoVE 60658

Two Flow Cytometric Approaches of NKG2D Ligand Surface Detection to Distinguish Stem Cells from Bulk Subpopulations in Acute Myeloid Leukemia

Henrik Landerer*¹, Marlon Arnone*¹, Ronja Wieboldt², Elsa Goersch¹, Anna M. Paczulla Stanger³, Martina Konantz¹, Claudia Lengerke^1,2,3

¹Department of Biomedicine, University Hospital Basel and University of Basel, ²Division for Hematology, University Hospital Basel and University of Basel, ³Department of Internal Medicine, Hematology and Oncology, University Hospital Tübingen

JoVE 61803

Patient-Derived Tumor Explants As a "Live" Preclinical Platform for Predicting Drug Resistance in Patients

Giuditta Viticchié¹, Ian Powley¹, Constantinos Demetriou¹, James Cooper¹, Michael Butterworth¹, Meeta Patel¹, Naila Abid¹, Gareth Miles¹, Lynne Howells¹, Howard Pringle¹, Marion MacFarlane², Catrin Pritchard¹

¹Leicester Cancer Research Center, University of Leicester, ²MRC Toxicology Unit

JoVE 62130

Methods Collection

Chemical Conjugation of a Purified DEC-205-Directed Antibody with Full-Length Protein for Targeting Mouse Dendritic Cells In Vitro and In Vivo

Julia Volckmar^1,2, Laura Knop^1,2,3, Tatjana Hirsch¹, Sarah Frentzel^1,2, Christian Erck⁴, Marco van Ham⁴, Sabine Stegemann-Koniszewski*^1,2,5, Dunja Bruder*^1,2

¹Immune Regulation Group, Helmholtz Centre for Infection Research, ²Infection Immunology Group, Institute of Medical Microbiology, Infection Prevention and Control, Health Campus Immunology, Infectiology and Inflammation, Otto-von-Guericke University Magdeburg, ³Institute for Molecular and Clinical Immunology, Health Campus Immunology, Infectiology and Inflammation, Otto-von-Guericke University Magdeburg, ⁴Cellular Proteome Research, Helmholtz Centre for Infection Research, ⁵Experimental Pneumology, University Hospital of Pneumology, Health Campus Immunology, Infectiology and Inflammation, Otto-von-Guericke University Magdeburg

JoVE 62018

Methods Collection

Modeling Brain Metastasis Via Tail-Vein Injection of Inflammatory Breast Cancer Cells

Xiaoding Hu*^1,2, Emilly S. Villodre*^1,2, Wendy A. Woodward^2,3, Bisrat G. Debeb^1,2

¹Department of Breast Medical Oncology, The University of Texas MD Anderson Cancer Center, ²Morgan Welch Inflammatory Breast Cancer Clinic and Research Program, The University of Texas MD Anderson Cancer Center, ³Department of Radiation Oncology, The University of Texas MD Anderson Cancer Center

JoVE 62249

Methods Collection

Discovery of Metastatic Regulators using a Rapid and Quantitative Intravital Chick Chorioallantoic Membrane Model

Konstantin Stoletov¹, Lian Willetts², Perrin H. Beatty¹, John D. Lewis¹

¹Department of Oncology, University of Alberta, Edmonton, Alberta, Canada, ²Department of Cell Biology & Anatomy, Cumming School of Medicine, University of Calgary

JoVE 62077

Methods Collection

Tumor Allotransplantation in Drosophila melanogaster with a Programmable Auto-Nanoliter Injector

Shangyu Gong*¹, Yichi Zhang*¹, Hongcun Bao¹, Xianfeng Wang¹, Chih-Hsuan Chang¹, Yi-Chun Huang¹, Wu-Min Deng¹

¹Department of Biochemistry and Molecular Biology, Tulane University School of Medicine

JoVE 62229

A Murine Ommaya Xenograft Model to Study Direct-Targeted Therapy of Leptomeningeal Disease

Vincent Law^1,2, Margi Baldwin³, Ganesan Ramamoorthi⁴, Krithika Kodumudi⁴, Nam Tran¹, Inna Smalley⁵, Derek Duckett⁶, Pawel Kalinski⁷, Brian Czerniecki⁴, Keiran S. M. Smalley², Peter A. Forsyth^1,2

¹Department of Neuro-Oncology, H. Lee Moffitt Cancer Center & Research Institute, ²Department of Tumor Biology, H. Lee Moffitt Cancer Center & Research Institute, ³Department of Comparative Medicine, University of South Florida, ⁴Department of Breast Oncology, H. Lee Moffitt Cancer Center & Research Institute, ⁵Department of Cancer Physiology, H. Lee Moffitt Cancer Center & Research Institute, ⁶Department of Drug Discovery, H. Lee Moffitt Cancer Center & Research Institute, ⁷Department of Medical Oncology, Roswell Park Comprehensive Cancer Center

JoVE 62033

A Three-dimensional Model of Spheroids to Study Colon Cancer Stem Cells

Maria Virginia Giolito^1,2, Léo Claret^1,2, Carla Frau¹, Michelina Plateroti^1,2

¹Département de la recherche, Centre de Recherche en Cancérologie de Lyon, INSERM U1052, CNRS UMR5286, Université de Lyon, Université Lyon 1, Centre Léon Bérard, ²UMR-S1113 - IRFAC INSERM, Université de Strasbourg

JoVE 61783

A Mouse Model to Investigate the Role of Cancer-associated Fibroblasts in Tumor Growth

David Jelinek^1,2, Ellen Ran Zhang^1,2,3, Aaron Ambrus^1,2, Erin Haley³, Emily Guinn^1,2, Austin Vo^1,2, Peter Le^1,2, Ayse Elif Kesaf^1,2, Jennifer Nguyen^1,2, Lily Guo^1,2, Destiny Frederick^1,2, Zhengyang Sun^1,2, Natalie Guo³, Parker Sevier^1,2, Eric Bilotta^1,2, Kaiser Atai^1,2,4, Laurent Voisin^1,2, Hilary A. Coller^1,2,4

¹Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, ²Department of Biological Chemistry, David Geffen School of Medicine, University of California, Los Angeles, ³Department of Molecular Biology, Princeton University, ⁴Molecular Biology Institute, University of California, Los Angeles

JoVE 61883

Methods Collection

Enhanced Viability for Ex vivo 3D Hydrogel Cultures of Patient-Derived Xenografts in a Perfused Microfluidic Platform

Lindsey K. Sablatura¹, Kristin M. Bircsak², Peter Shepherd³, Karla Queiroz⁴, Mary C. Farach-Carson^1,5,6, Pamela E. Constantinou^1,7, Anthony Saleh², Nora Navone³, Daniel A. Harrington^1,5,6

¹Department of BioSciences, Rice University, ²Mimetas US Inc, ³Department of Genitourinary Medical Oncology Research, Division of Cancer Medicine, The University of Texas MD Anderson Cancer Center, ⁴Mimetas B.V., ⁵Department of Bioengineering, Rice University, ⁶Department of Diagnostic and Biomedical Sciences, The University of Texas Health Science Center, ⁷Sheikh Ahmed Center for Pancreatic Cancer Research, The University of Texas MD Anderson Cancer Center

JoVE 60872

High-Throughput Cellular Profiling of Targeted Protein Degradation Compounds using HiBiT CRISPR Cell Lines

Kristin M. Riching¹, Sarah D. Mahan¹, Marjeta Urh¹, Danette L. Daniels¹

¹Promega Corporation

JoVE 61787

Isolation of Proximal Fluids to Investigate the Tumor Microenvironment of Pancreatic Adenocarcinoma

Greta Donisi¹, Marialuisa Barbagallo², Giovanni Capretti^1,3, Gennaro Nappo^1,3, Panteleimon G. Takis^4,5,6, Alessandro Zerbi^1,3, Federica Marchesi^2,7, Nina Cortese²

¹Section of Pancreatic Surgery, Humanitas Clinical and Research Center-IRCCS, ²Department of Immunology and Inflammation, Humanitas Clinical and Research Center-IRCCS, ³Department of Biomedical Sciences, Humanitas University, ⁴Giotto Biotech S.R.L., ⁵Section of Bioanalytical Chemistry, Division of Systems Medicine, Department of Metabolism, Digestion and Reproduction, Imperial College London, ⁶National Phenome Centre, Department of Metabolism, Digestion and Reproduction, Faculty of Medicine, Imperial College London, ⁷Department of Medical Biotechnology and Translational Medicine, University of Milan

JoVE 61687

Studying TGF-β Signaling and TGF-β-induced Epithelial-to-mesenchymal Transition in Breast Cancer and Normal Cells

Jing Zhang¹, Midory Thorikay¹, Gerard van der Zon¹, Maarten van Dinther¹, Peter ten Dijke¹

¹Oncode Institute and Department of Cell Chemical Biology, Leiden University Medical Center

JoVE 61830

Establishing a Competing Risk Regression Nomogram Model for Survival Data

Lunpo Wu^1,2, Chenyang Ge³, Hongjuan Zheng⁴, Haiping Lin⁵, Wei Fu⁶, Jianfei Fu⁴

¹Department of Gastroenterology, Second Affiliated Hospital of Zhejiang University School of Medicine, ²Institute of Gastroenterology, Zhejiang University, ³Department of Colorectal Surgery, Afiliated Jinhua Hospital, Zhejiang University School of Medicine, ⁴Department of Medical Oncology, Affiliated Jinhua Hospital, Zhejiang University School of Medicine, ⁵Department of Hepatobiliary Pancreatic Surgery, Affiliated Jinhua Hospital, Zhejiang University School of Medicine, ⁶Division of Oncology, Johns Hopkins University School of Medicine

JoVE 60684

Identification of EGFR and RAS Inhibitors using Caenorhabditis elegans

Dharini van der Hoeven^1,2, Thuy Nhu L. Truong¹, Ali Naji¹, Sabita Thapa¹, John F. Hancock², Ransome van der Hoeven¹

¹Department of Diagnostic and Biomedical Sciences, School of Dentistry, University of Texas Health Science Center, ²Department of Integrative Biology and Pharmacology, McGovern Medical School, University of Texas Health Science Center

JoVE 61788

Using Computer-based Image Analysis to Improve Quantification of Lung Metastasis in the 4T1 Breast Cancer Model

Margaret A. Nagai-Singer¹, Alissa Hendricks-Wenger^1,2,3, Rebecca M. Brock^1,2, Holly A. Morrison¹, Juselyn D. Tupik¹, Sheryl Coutermarsh-Ott¹, Irving C. Allen^1,2,4

¹Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine, ²Graduate Program in Translational Biology, Medicine and Health, Virginia Polytechnic Institute and State University, ³Department of Biomedical Engineering and Mechanics, Virginia Polytechnic Institute and State University, ⁴Department of Basic Science Education, Virginia Tech Carilion School of Medicine, Virginia Polytechnic Institute and State University

JoVE 61805

Methods Collection

Isolation and Functional Assessment of Human Breast Cancer Stem Cells from Cell and Tissue Samples

Vasudeva Bhat^1,2, Cory Lefebvre^1,2, David Goodale¹, Mauricio Rodriguez-Torres^1,2, Alison L. Allan^1,2,3,4

¹London Regional Cancer Program, ²Department of Anatomy & Cell Biology, Western University, ³Department of Oncology, Western University, ⁴Lawson Health Research Institute

JoVE 61775

An Orthotopic Resectional Mouse Model of Pancreatic Cancer

Tony C. Y. Pang^1,2,4,5, Zhihong Xu^1,2, Alpha Raj Mekapogu^1,2, Srinivasa Pothula^1,2, Therese M. Becker³, David Goldstein¹, Romano C. Pirola^1,2, Jeremy S. Wilson^1,2, Minoti V. Apte^1,2

¹Pancreatic Research Group, South Western Sydney Clinical School, University of New South Wales, ²Ingham Institute for Applied Medical Research, ³Centre for Circulating Tumour Cell Diagnostics and Research, Ingham Institute for Applied Medical Research, ⁴Surgical Innovations Unit, Westmead Hospital, ⁵Westmead Clinical School, University of Sydney