JoVE Journal

Cancer Research

JoVE Cancer Research includes experimental approaches and methods to understand, detect, treat, and prevent cancer.

1 2 3 4 5 6 7 8 9 10 › 233 VIDEO ARTICLES

A Human Peripheral Blood Mononuclear Cell (PBMC) Engrafted Humanized Xenograft Model for Translational Immuno-oncology (I-O) Research

Zhuo Li*¹, Xiao Yang*¹, Yilu Zhang¹, Xiaolong Yang¹, Xinxin Cui¹, Yanjuan Zhang¹, Wenfeng Gong¹, Huichen Bai¹, Ning Liu¹, Zhiyu Tang¹, Mingming Guo¹, Kang Li¹, Tong Zhang¹, Lai Wang¹, Xiaomin Song¹

¹BeiGene (Beijing) Co., Ltd.

Semi-automatic PD-L1 Characterization and Enumeration of Circulating Tumor Cells from Non-small Cell Lung Cancer Patients by Immunofluorescence

Jessica Garcia^1,2,3, David Barthelemy^1,2,3, Florence Geiguer^1,2, Julie Ballandier^1,2,3, Kathryn W. Li⁴, Jean-Phillippe Aurel⁴, Frédérique Le Breton⁵, Claire Rodriguez-Lafrasse¹, Brigitte Manship³, Sébastien Couraud^6,7, Léa Payen^1,2,3

¹Laboratoire de Biochimie et Biologie Moléculaire, Groupe Hospitalier Sud, Hospices Civils de Lyon, ²Circulating Cancer (CIRCAN) Program, Hospices Civils de Lyon Cancer Institute, ³University of Lyon, Claude Bernard University, Cancer Research Center of Lyon, ⁴Biolidics Limited, ⁵Institut de Pathologie Multisites des HCL-Site Sud, Hospices Civils de Lyon, ⁶Acute Respiratory Disease and Thoracic Oncology Department, Lyon Sud Hospital, Hospices Civils de Lyon Cancer Institute, ⁷EMR-3738 Therapeutic Targeting in Oncology, Lyon Sud Medical Faculty, Lyon 1 University

In Vivo Imaging and Quantitation of the Host Angiogenic Response in Zebrafish Tumor Xenografts

Denver D. Britto¹, Christopher J. Hall¹, Jonathan W. Astin¹

¹Department of Molecular Medicine and Pathology, School of Medical Sciences, University of Auckland

A Three-dimensional Thymic Culture System to Generate Murine Induced Pluripotent Stem Cell-derived Tumor Antigen-specific Thymic Emigrants

Raul Vizcardo*^1,2, S.M. Rafiqul Islam*^1,2, Takuya Maeda^1,2, Naritaka Tamaoki^1,2, Meghan L. Good^1,2, Nicholas D. Klemen^1,2, Marta Bosch-Marce^1,2, Li Jia¹, Mikhael J. Kruhlak³, Nicholas P. Restifo^1,2

¹Surgery Branch, National Cancer Institute, NIH, ²Center for Cell-Based Therapy, National Cancer Institute, NIH, ³Laboratory of Cancer Biology and Genetics, Center for Cancer Research, National Cancer Institute, NIH

A 3D Spheroid Model as a More Physiological System for Cancer-Associated Fibroblasts Differentiation and Invasion In Vitro Studies

Ana C. Martins Cavaco^1,2, Johannes A. Eble¹

¹Institute of Physiological Chemistry and Pathobiochemistry, University of Münster, ²Instituto de Medicina Molecular, Lisbon

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Paola Monti¹, Bartolomeo Bosco², Sara Gomes³, Lucilia Saraiva*³, Gilberto Fronza*¹, Alberto Inga*²

¹Mutagenesis and Cancer Prevention Unit, Ospedale Policlinico San Martino, ²Department CIBIO, University of Trento, ³LAQV/REQUIMTE, Laboratory of Microbiology, Faculty of Pharmacy, University of Porto

In Vitro Assay to Study Tumor-macrophage Interaction

Zhenyi An¹, William A. Weiss^1,2,3

¹Department of Neurology, University of California, San Francisco, ²Helen Diller Family Comprehensive Cancer Center, University of California, San Francisco, ³Departments of Pediatrics and Neurological Surgery, University of California, San Francisco

E-Patient Counseling Trial (E-PACO): Computer Based Education versus Nurse Counseling for Patients to Prepare for Colonoscopy

Govert Veldhuijzen¹, Aura A. van Esch¹, Michael Klemt-Kropp², Jochim S. Terhaar sive Droste³, Joost P.H. Drenth¹

¹Department of Gastroenterology and Hepatology, Radboud University Medical Center, ²Department of Gastroenterology and Hepatology, North West Clinics, ³Department of Gastroenterology and Hepatology, Jeroen Bosch Hospital

Detection of Retrotransposition Activity of Hot LINE-1s by Long-Distance Inverse PCR

Barun Pradhan^1,2, Liisa Kauppi^1,2

¹Systems Oncology Research Program, University of Helsinki, ²Department of Biochemistry and Developmental Biology, Medicum, University of Helsinki

Optimization, Design and Avoiding Pitfalls in Manual Multiplex Fluorescent Immunohistochemistry

Jenny Lazarus¹, Yagiz Akiska¹, Mirna Perusina Lanfranca¹, Lawrence Delrosario¹, Lei Sun¹, Daniel Long², Jiaqi Shi³, Howard Crawford², Marina P. Di Magliano¹, Weiping Zou¹, Timothy Frankel¹

¹Department of Surgery, University of Michigan, ²Department of Molecular and Cellular Physiology, University of Michigan, ³Department of Pathology, University of Michigan

Studying Normal Tissue Radiation Effects using Extracellular Matrix Hydrogels

Steven M Alves¹, Tian Zhu¹, Anastasia Shostak¹, Ninna S. Rossen², Marjan Rafat^1,3,4

¹Department of Chemical and Biomolecular Engineering, Vanderbilt University, ²Department of Radiation Oncology, Stanford University, ³Depattment of Biomedical Engineering, Vanderbilt University, ⁴Department of Radiation Oncology, Vanderbilt University Medical Center

Using CRISPR/Cas9 to Knock Out GM-CSF in CAR-T Cells

Rosalie M. Sterner^1,2, Michelle J. Cox³, Reona Sakemura³, Saad S. Kenderian^2,3

¹Mayo Clinic Medical Scientist Training Program, Mayo Clinic College of Medicine and Science, ²Department of Immunology, Mayo Clinic, ³Division of Hematology, Mayo Clinic

Transfer of Manipulated Tumor-associated Neutrophils into Tumor-Bearing Mice to Study their Angiogenic Potential In Vivo

Ekaterina Pylaeva¹, Ilona Spyra¹, Sharareh Bordbari¹, Stephan Lang¹, Jadwiga Jablonska¹

¹Department of Otorhinolaryngology, University Hospital, University of Duisburg-Essen

Establishment of Gastric Cancer Patient-derived Xenograft Models and Primary Cell Lines

Jia-huan Lu*¹, Yun Wang*¹, Qi Meng*¹, Zhao-lei Zeng¹

¹State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center

Characterize Disease-related Mutants of RAF Family Kinases by Using a Set of Practical and Feasible Methods

Jiajun Yap¹, Jimin Yuan¹, Zi Heng Tee¹, Xuchao Huang¹, Wan Hwa Ng¹, Jiancheng Hu^1,2

¹The Laboratory of Cancer Signaling, Division of Cellular and Molecular Research, National Cancer Centre Singapore, ²The Cancer and Stem Cell Program, Duke-NUS Medical School

Oncogenic Gene Fusion Detection Using Anchored Multiplex Polymerase Chain Reaction Followed by Next Generation Sequencing

Michael Seager¹, Dara L. Aisner¹, Kurtis D. Davies¹

¹Department of Pathology, University of Colorado - Anschutz Medical Campus

Physiologic Patient Derived 3D Spheroids for Anti-neoplastic Drug Screening to Target Cancer Stem Cells

Michael E. Bregenzer*¹, Ciara Davis*¹, Eric N. Horst*¹, Pooja Mehta*², Caymen M. Novak*¹, Shreya Raghavan*², Catherine S. Snyder*², Geeta Mehta^1,2,3,4

¹Department of Biomedical Engineering, University of Michigan, ²Department of Materials Science and Engineering, University of Michigan, ³Rogel Cancer Center, School of Medicine, University of Michigan, ⁴Macromolecular Science and Engineering, University of Michigan

Assessing Tumor Microenvironment of Metastasis Doorway-Mediated Vascular Permeability Associated with Cancer Cell Dissemination using Intravital Imaging and Fixed Tissue Analysis

George S. Karagiannis^1,2,3, Jessica M. Pastoriza^1,2,4, Lucia Borriello^1,2, Rojin Jafari^1,2, Anouchka Coste^1,2,4, John S. Condeelis^1,2,3,4, Maja H. Oktay^1,2,3,5, David Entenberg^1,2,3

¹Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, ²Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, ³Integrated Imaging Program, Albert Einstein College of Medicine, ⁴Department of Surgery, Montefiore Medical Center, ⁵Department of Pathology, Montefiore Medical Center

Establishment and Characterization of Three Afatinib-resistant Lung Adenocarcinoma PC-9 Cell Lines Developed with Increasing Doses of Afatinib

Toshimitsu Yamaoka¹, Motoi Ohba¹, Yuki Matsunaga², Junji Tsurutani¹, Tohru Ohmori³

¹Advanced Cancer Translational Research Institute, Showa University, ²Division of Breast Surgical Oncology, Department of Surgery, Showa University School of Medicine, ³Division of Allergology and Respiratory Medicine, Department of Medicine, Showa University School of Medicine

Rapid Generation of Primary Murine Melanocyte and Fibroblast Cultures

Brandon M. Murphy¹, Tirzah J. Weiss¹, Christin E. Burd^1,2

¹Department of Cancer Biology and Genetics, The Ohio State University, ²Department of Molecular Genetics, The Ohio State University

Assessing Cell Viability and Death in 3D Spheroid Cultures of Cancer Cells

Michala G. Rolver¹, Line O. Elingaard-Larsen¹, Stine F. Pedersen¹

¹Section for Cell Biology and Physiology, Department of Biology, Faculty of Science, University of Copenhagen

Isolation and Characterization of Tumor-initiating Cells from Sarcoma Patient-derived Xenografts

Dan Han¹, Veronica Rodriguez-Bravo¹, Sudeh Izadmehr¹, Josep Domingo-Domenech¹, Carlos Cordon-Cardo¹

¹Department of Pathology, Icahn School of Medicine at Mount Sinai

Extraction of Aqueous Metabolites from Cultured Adherent Cells for Metabolomic Analysis by Capillary Electrophoresis-Mass Spectrometry

Ami Maruyama^1,2, Kenjiro Kami³, Kazunori Sasaki³, Hajime Sato³, Yuzo Sato^1,2, Katsuya Tsuchihara⁴, Hideki Makinoshima^1,2,4

¹Shonai Regional Industry Promotion Center, ²Tsuruoka Metabolomics Laboratory, National Cancer Center, ³Human Metabolome Technologies, Inc, ⁴Division of Translational Informatics, Exploratory Oncology Research and Clinical Trial Center, National Cancer Center

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids

Erin R. Bonner^1,2, Karim Saoud¹, Sulgi Lee^1,2, Eshini Panditharatna³, Madhuri Kambhampati¹, Sabine Mueller^4,5, Javad Nazarian^1,2,5

¹Center for Genetic Medicine, Children's National Health System, ²Institute for Biomedical Sciences, The George Washington University School of Medicine and Health Sciences, ³Dana-Farber Cancer Institute, ⁴Department of Neurology, University of California San Francisco, ⁵DIPG Centre of Expertise Zurich, Universitats-Kinderspital Zurich

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