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Journal
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Biology
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gentleMACS Dissociator从小鼠脾脏单细胞悬浮液的制备
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Preparation of Single-Cell Suspensions from Mouse Spleen with the gentleMACS Dissociator
Please note that all translations are automatically generated.
Click here for the English version.
gentleMACS Dissociator从小鼠脾脏单细胞悬浮液的制备
DOI:
10.3791/1029-v
•
05:18 min
•
December 11, 2008
•
Melanie Jungblut
,
Karen Oeltze
,
Irene Zehnter
,
Doris Hasselmann
,
Andreas Bosio
1
Miltenyi Biotec,GmbH
Chapters
00:00
Title
00:26
Introduction
00:58
Introduction to Equipment
01:55
Dissociating the spleen using the gentleMACS Dissociator
03:42
Filtration of dissociated spleen
04:49
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本视频介绍了一个简单的自动组织使用gentleMACS Dissociator准备小鼠脾单细胞悬液的解离,节省时间的技术。
Tags
Single-Cell Suspensions
Mouse Spleen
GentleMACS Dissociator
Experiments
Cell Separation
Cell Analysis
Cell Culture
Manual Dissociation
Controlled Conditions
Reproducible Conditions
User-safety
Video Tutorial
Automated Device
Viable Cell Suspensions
Filtering
Centrifugation
Resuspension
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