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gentleMACS Dissociatorとマウス脾臓からの単細胞懸濁液の調製
JoVE Journal
Biology
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JoVE Journal
Biology
Preparation of Single-Cell Suspensions from Mouse Spleen with the gentleMACS Dissociator
Please note that all translations are automatically generated.
Click here for the English version.
gentleMACS Dissociatorとマウス脾臓からの単細胞懸濁液の調製
DOI:
10.3791/1029-v
•
05:18 min
•
December 11, 2008
•
Melanie Jungblut
,
Karen Oeltze
,
Irene Zehnter
,
Doris Hasselmann
,
Andreas Bosio
1
Miltenyi Biotec,GmbH
Chapters
00:00
Title
00:26
Introduction
00:58
Introduction to Equipment
01:55
Dissociating the spleen using the gentleMACS Dissociator
03:42
Filtration of dissociated spleen
04:49
Conclusion
Summary
Automatic Translation
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Automatic Translation
このビデオでは、マウス脾細胞の単細胞懸濁液を準備するためにgentleMACSのDissociatorを使用して自動化された組織の解離のためのシンプルな、時間の節約テクニックを説明します。
Tags
Single-Cell Suspensions
Mouse Spleen
GentleMACS Dissociator
Experiments
Cell Separation
Cell Analysis
Cell Culture
Manual Dissociation
Controlled Conditions
Reproducible Conditions
User-safety
Video Tutorial
Automated Device
Viable Cell Suspensions
Filtering
Centrifugation
Resuspension
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