JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
光学测绘的Langendorff灌注大鼠心脏
JoVE Journal
Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biology
Optical Mapping of Langendorff-perfused Rat Hearts
Please note that all translations are automatically generated.
Click here for the English version.
光学测绘的Langendorff灌注大鼠心脏
DOI:
10.3791/1138-v
•
11:48 min
•
August 11, 2009
•
Bjoern Sill
,
Peter E. Hammer
2
,
Douglas B. Cowan
1
Department of Anesthesiology, Perioperative and Pain Medicine
,
Children’s Hospital Boston and Harvard Medical School
,
2
Departments of Cardiac Surgery
,
Children’s Hospital Boston and Harvard Medical School
Chapters
00:05
Title
00:11
Introduction
00:41
Preparing the Solutions and the Langendorff System
02:01
Harvesting of the Rat Heart and Set-up of the Langendorff Perfusion System
04:55
Loading Potentiometric Dye and Acquiring Data
08:45
Analyze Acquisition Information Using RedShirt Imaging Software
10:38
Representative Visualization of a Myocardial Excitation Wave
11:08
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
本文介绍了高时空分辨率技术的光学图像行动的Langendorff灌注大鼠的心,用一个电位染料(DI - 8 - ANEPPS)表面上的潜在流动。
Tags
Optical Mapping
Langendorff-perfused Rat Hearts
Voltage-sensitive Fluorescent Dyes
Electrical Excitation
Experimental Models
Action Potentials
Arrhythmias
Spatiotemporal Resolutions
Custom-built Equipment
Electrophysiological Methods
Complementary Metal-oxide-semiconductor (CMOS) Cameras
Light-emitting Diodes (LEDs)
Voltage-sensitive Dyes
Optics
Filters
Cardiovascular Cell Biologists
Microscopes
Fluorescence Acquisition Technologies
Photodiode Arrays
Laser Scanning Systems
Cooled Charged-coupled Device (CCD) Cameras
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
适当的照顾和清洁的显微镜
光镜下的主要组成部分
相衬,微分干涉对比(DIC)显微镜
半自动小红桃光学心跳分析
单细胞在斑马鱼的命运映射
细胞标记和注射胚胎发展小鼠心脏
恢复成年斑马鱼的心脏的高通量应用
培养HL-1心房肌细胞单层的电压和钙双通道光映射
的内肌浆网钙光学标测<sup> 2+</sup>和的Langendorff灌注兔心脏跨膜电位
鼠标窦房结的高分辨率光学测绘
Read Article