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Chapters
- 00:00Title
- 00:56Introduction
- 01:25‘Inside-Out’ Tissue Preparation
- 04:29Neuronal Bouton Labeling with Fluorescently Labeled Primary Antibody Against HRP
- 05:23Perisynaptic Space Labeling with Dextran Fluorophore Conjugate
- 06:12Mounting the Tissue for Visualization Under a Confocal Microscope
- 07:23Representative Results
- 10:17Conclusion
Nous avons décrit les caractéristiques structurelles des synapses neuromusculaires Glia-dans un roman de préparation des tissus intérieur-extérieur de larves de mouches en direct en utilisant des colorants fluorescents en microscopie confocale. Nous avons étiqueté terminaisons neuronales vivre avec fluorescentes anticorps primaires à HRP, et également visualisé l'espace perisynaptic avec dextranes fluorescents.
Tags
VisualizingLiveDrosophilaGlial-neuromuscular JunctionFluorescent DyesGFP Labeled Glial StructuresDeveloping Larval Fly Neuromuscular SynapseLive Glial-nerve-muscle SynapsesOptical PropertiesAccess Of PerfusatesFly LarvaeArtificial HemolymphRhythmic Body ContractionsDissectionPosterior SegmentsBlunt Insect PinEverted Larval Body WallUltra-fine Dissection ScissorsVisceral Side Of Body Wall MusclesGlial Structures At NMJMembrane Targeted GFPGlial Specific PromotersPost-synaptic MembraneSSR (Subsynaptic Reticula)Muscle Expressed Synaptically Targeted DsRedAcutely Label Motor Neuron TerminalsPrimary Antibodies To HRPDye Diffusion PropertiesPerisynaptic Space
