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Canlı görselleştirme Drosophila Glial nöromüsküler Kavşağı
JoVE Journal
Biology
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JoVE Journal Biology
Visualizing the Live Drosophila Glial-neuromuscular Junction with Fluorescent Dyes
DOI:

10:53 min

May 13, 2009

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Chapters

  • 00:00Title
  • 00:56Introduction
  • 01:25‘Inside-Out’ Tissue Preparation
  • 04:29Neuronal Bouton Labeling with Fluorescently Labeled Primary Antibody Against HRP
  • 05:23Perisynaptic Space Labeling with Dextran Fluorophore Conjugate
  • 06:12Mounting the Tissue for Visualization Under a Confocal Microscope
  • 07:23Representative Results
  • 10:17Conclusion

Summary

Automatic Translation

Biz sinek larvaları konfokal mikroskobu ile floresan boyalar kullanarak canlı bir roman Tersyüz doku hazırlanması Glia nöromüsküler sinapsların yapısal özellikleri nitelendirdi. Biz HRP için flüoresan primer antikor ile canlı nöron terminalleri etiketli ve floresan Dextrans perisynaptic alan görüntülendi.

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